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TSKgel® Hhr Columns

TSKgel® HHR Columns for Expanded Solvent Capability

The TSKgel® HHR column line consists of eight columns with different pore sizes, TSKgel® G1000HHR through TSKgel® G7000HHR, and five columns with an extended linear range of the calibration curve. The calibration curves are shown below.

Main characteristics

  • Maximum operating temperatures for TSKgel® HHR columns is 140 °C for the conventional bed columns and 80 °C for the mixed bed columns. In addition, nine TSKgel® HHR columns are available for high temperature and ultra-high temperature analysis, with maximum operating temperatures of 140 °C and 220 °C, respectively.
  • Broad solvent range. All TSKgel® HHR columns are shipped in THF, which can be exchanged for a wide variety of organic solvents as shown below.
  • TSKgel® HHR columns are conventional GPC columns with dimensions of 7.8 mm ID x 30 cm. Most HPLC systems do not require optimization of extra-column band broadening effects when working with conventional GPC columns.

Columns with linear calibration curves

The TSKgel® HHR product line contains five mixed bed columns, in which particles with different pore sizes are blended to provide an extended linear calibration curve. The mixed bed columns feature increasing linear calibration ranges, from TSKgel® GMHHR-L, GMHHR-N, GMHHR-M, to GMHHR-H.

The calibration curves are shown below for all TSKgel® HHR columns.

Calibration Curves on TSKgel® HHR Columns

Shipping Solvent

Can Be Replaced With
Tetrahydrofuran 2,3 

acetone, ethanol, quinoline, benzene, o-dichlorobenzene, ethyl acetate, 

dodecane, FC-113, carbon tetrachloride, dichloromethane, 

dichloroethane, trichloroethane, n-hexane, cyclohexane, 

xylene, chloroform, 1,4-dioxane, hexafluoroisopropanol, toluene,
1-chloronaphthalene, N,N-dimethylacetoacetamide, methyl ethyl ketone,
trichlorobenzene, m-cresol/chloroform, dimethylformamide, N-methylpyrrolidone, 

o-chlorophenol/chloroform, dimethyl sulfoxide, pyridine, 
hexafluoroisopropanol/chloroform, methanol/chloroform

Notes:

  • See table below for appropriate flow rates for solvent exchange. After switching to a very polar solvent, switching to a nonpolar is not recommended.
  • All TSKgel® HHR analytical columns are shipped containing tetrahydrofuran (THF), except high temperature (HT) columns, which contain o-dichlorobenzene.
  • Prolonged exposure to carbon tetrachloride can corrode the stainless steel parts of a column and an HPLC system.

* 100% methanol cannot be used with TSKgel® H-type columns; use this solvent with TSKgel® SW-type or Alpha columns.
 

Solvent

Recommended Flow Rate
n-Hexane 0.9
methyl ethyl ketone 0.7
dichloromethane, ethyl acetate
0.6
toluene, chloroform 0.5
dimethylformamide 0.4
carbon tetrachloride, pyridine  0.3
dimethyl sulfoxide, dioxane, ethanol,
N-methylpyrrolidone, o-dichlorobenzene		  0.2
quinoline, hexafluoroisopropanal,
1-chloronaphthalene		 0.1



Recommended Solvent

Application
THF polystyrene, epoxy resin, phenoxy resin, polycarbonate, 
polyisoprene, polyvinyl acetate, polyvinyl chloride, 
monoglycerides, fatty acids, poly(butadiene), 
polybutadiene, poly(methyl methacrylate), 
poly(styrene-butadiene), poly(styrene-acrylonitrile)
N,N-Dimethylformamide (DMF) +/- 5mM LiBr polyvinyl chloride, polyvinyl fluoride, urea resins, 
polyurethane, polystyrene, polyester, 
polyimido ether, polyimido ester, 
polyphenol (aqueous solution), polyacrylonitrile
o-Dichlorobenzene (ODCB)
polyethylene, polypropylene
Chloroform polycarboxylic ether, acrylic resin, 
epoxy resin, polystyrene
m-Cresol/Chloroform nylon, polyester, polyamide, 
poly(ethylene terephthalate)
Toluene  polybutadiene, polysiloxane



Matrix: polystyrene-divinylbenzene  
Particle Size (mean): 5 µm  
Pore size (mean): 1.5 nm  
Exclusion Limit: < 1,000 Da             Visit the TSKgel® G1000HHR product page.
Max. Temp. (ºC): 140           
Shipping Solvent: THF

   

Matrix: polystyrene-divinylbenzene  
Particle Size (mean): 5 µm  
Pore size (mean): 2 nm  
Exclusion Limit: < 10,000 Da              Visit the TSKgel® G2000HHR product page.
Max. Temp. (ºC): 140           
Shipping Solvent: THF

   

Matrix: polystyrene-divinylbenzene  
Particle Size (mean): 5 µm  
Pore size (mean): 3 nm  
Exclusion Limit: 20,000 Da             Visit the TSKgel® G2500HHR product page.
Max. Temp. (ºC): 140           
Shipping Solvent: THF

   

Matrix: polystyrene-divinylbenzene  
Particle Size (mean): 5 µm  
Pore size (mean): 7.5 nm  
Exclusion Limit: < 60,000 Da             Visit the TSKgel® G3000HHR product page.
Max. Temp. (ºC): 140           
Shipping Solvent: THF

   

Matrix: polystyrene-divinylbenzene  
Particle Size (mean): 5 µm  
Pore size (mean): 20 nm  
Exclusion Limit: < 400,000 Da             Visit the TSKgel® G4000HHR product page.
Max. Temp. (ºC): 140           
Shipping Solvent: THF

   

Matrix: polystyrene-divinylbenzene  
Particle Size (mean): 5 µm  
Pore size (mean): 65 nm  
Exclusion Limit: < 4 x 106 Da             Visit the TSKgel® G5000HHR product page.
Max. Temp. (ºC): 140           
Shipping Solvent: THF

   

Matrix: polystyrene-divinylbenzene  
Particle Size (mean): 5 µm  
Pore size (mean): ND  
Exclusion Limit: < 4 x 107 Da             Visit the TSKgel® G6000HHR product page.
Max. Temp. (ºC): 140           
Shipping Solvent: THF

   

Matrix: polystyrene-divinylbenzene  
Particle Size (mean): 5 µm  
Pore size (mean): ND  
Exclusion Limit: 4 x 108 Da             Visit the TSKgel® G7000HHR product page.
Max. Temp. (ºC): 140           
Shipping Solvent: THF

   

Matrix: polystyrene-divinylbenzene  
Particle Size (mean): 5 µm  
Pore size (mean): ND  
Exclusion Limit: < 4 x 108 Da             Visit the TSKgel® GMHHR-H mixed-bed product page.
Max. Temp. (ºC): 140           
Shipping Solvent: THF

   

Matrix: polystyrene-divinylbenzene  
Particle Size (mean): 13 µm  
Pore size (mean): mixed  
Exclusion Limit: < 4 x 108 Da    Visit the TSKgel® GMHHR-H(S) HT mixed bed product page.
Max. Temp. (ºC): 140           
Shipping Solvent: ODCB

   

Matrix: polystyrene-divinylbenzene  
Particle Size (mean): 5 µm  
Pore size (mean): mixed  
Exclusion Limit: 4 x 106 Da             Visit the TSKgel® GMHHR-L product page.
Max. Temp. (ºC): 80           
Shipping Solvent: THF

   

Matrix: polystyrene-divinylbenzene  
Particle Size (mean): 5 and 13 µm  
Pore size (mean): mixed  
Exclusion Limit: < 4 x 106 Da             Visit the TSKgel® GMHHR-M product page.
Max. Temp. (ºC): 80           
Shipping Solvent: THF

   

Matrix: polystyrene-divinylbenzene  
Particle Size (mean): 5 µm  
Pore size (mean): mixed  
Exclusion Limit: < 400,000 Da             Visit the TSKgel® GMHHR-N product page.
Max. Temp. (ºC): 80           
Shipping Solvent: THF