TSKgel® HXL Columns for Reduced Polymer Adsorption and Limited Solvent Range
The TSKgel® HXL column lines consists of eight columns with different pore sizes, TSKgel® G1000HXL through TSKgel® G7000HXL, and three columns with an extended linear range of the calibration curve, TSKgel® GMHXL, TSKgel® GMHXL-L and TSKgel® MultiporeHXL-M. The calibration curves for all TSKgel® HXL columns are shown.
Main characteristics
- Ultra-low sample adsorption, i.e., the columns show true size exclusion behavior for most polymers
- Maximum operating temperatures for TSKgel® HXL columns is 60 °C for G1000HXL, G2000HXL, G2500HXL and G3000HXL, and 80 °C for the remaining columns in the product line. One of the mixed bed columns is available for high temperature analysis up to 140 °C.
- Limited solvent range. The columns are shipped in THF, which can be exchanged for a limited number of organic solvents (see Table below).
- TSKgel® HXL columns are conventional GPC columns with column dimensions of 7.8 mm ID x 30 cm. Most HPLC systems do not require optimization of extra-column bandbroadening effects when working with conventional GPC columns.
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Calibration Curves for TSKgel® HXL Columns with Polystyrene Standards
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TSKgel® HXL columns with linear calibration curves
Two of the TSKgel® HXL columns are mixed bed columns, in which particles with different pore sizes are blended to provide an extended linear calibration curve. The TSKgel® MultiporeXL-M column is packed with particles containing a wide range of pore sizes that provide a linear calibration curve. The unique multi-pore approach, pioneered by Tosoh, is a synthetic chemistry answer to the question of how to obtain a column with an extended linear calibration curve, while mixed bed columns represent a mechanical way of obtaining a linear calibration curve. In general, Multipore columns have a smoother, more linear, calibration curve. See the product page for multi-pore technology for the principles behind mixed bed and multi-pore columns.
Solvent compatibility for TSKgel® HXL columns
The column shipping solvent limits the types of solvents that can be used with each column type.
Shipping Solvent
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Can Be Replaced With
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| Tetrahydrofuran 2,3 |
benzene, chloroform, toluene, xylene,
dichloromethane, dichloroethane
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| Acetone** |
carbon tetrachloride4, o-dichlorobenzene, dimethylformamide,
dodecane, dimethyl sulfoxide, 1,4-dioxane, ethylacetate,
FC-113 hexafluoroisopropanol/chloroform,
methyl ethyl ketone, quinoline, cyclohexane, N-methylpyrrolidone
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| Chloroform** |
m-cresol in chloroform, up to 10%
hexafluoroisopropanol in chloroform
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| Dimethylformamide** |
dimethyl sulfoxide, dioxane, tetrahydrofuran, toluene |
- In case of TSKgel® HXL, keep flow rate below <0.5 mL/min during solvent change. Solvent can be changed one way/one time only.
- All TSKgel® HXL analytical columns are shipped containing tetrahydrofuran (THF), except TSKgel® GMH-HT columns, which contain o-dichlorobenzene.
- THF in TSKgel® G1000HXL columns cannot be replaced with dichloromethane or dichloroethane.
- Prolonged exposure to carbon tetrachloride can corrode the stainless steel parts of a column and an HPLC system.
* 100% methanol cannot be used with TSKgel® H-type columns; use this solvent with TSKgel® SW-type or Alpha columns.
** TSKgel® H columns may be specially ordered with this shipping solvent.
Recommended Solvent
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Application
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| THF |
polystyrene, epoxy resin, phenoxy resin, polycarbonate,
polyisoprene, polyvinyl acetate, polyvinyl chloride,
monoglycerides, fatty acids, poly(butadiene),
polybutadiene, poly(methyl methacrylate),
poly(styrene-butadiene), poly(styrene-acrylonitrile) |
| N,N-Dimethylformamide (DMF) +/- 5mM LiBr |
polyvinyl chloride, polyvinyl fluoride, urea resins,
polyurethane, polystyrene, polyester,
polyimido ether, polyimido ester,
polyphenol (aqueous solution), polyacrylonitrile |
| o-Dichlorobenzene (ODCB) |
polyethylene, polypropylene
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| Chloroform |
polycarboxylic ether, acrylic resin,
epoxy resin, polystyrene |
| m-Cresol/Chloroform |
nylon, polyester, polyamide,
poly(ethylene terephthalate) |
| Toluene |
polybutadiene, polysiloxane |