TSKgel BioAssist Q

TSKgel BioAssist Q columns are packed with polymethacrylate particles containing very large pores (~400 nm) that are functionalized with polyamine groups to form a network structure. The very large pores allow large biomolecules to freely enter and exit the pores resulting in higher binding capacity and better recovery of activity than other competitive materials. The capacity of the TSKgel BioAssist Q columns is high over a wide molecular weight range (up to 1.0 × 10⁶ Da).

The standard TSKgel BioAssist Q column is 4.6 mm ID × 5 cm, while a 10 mm ID × 10 cm semi-preparative column is available for scale-up. Both columns are made of PEEK to reduce protein adsorption.

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0019685	TSKgel BioAssist Q	10	PEEK	4.6	5	1,756	Inquire
0021410	TSKgel BioAssist Q	13	PEEK	10	10	6,686	Inquire

Product Attributes

Matrix:	polymethacrylate
Particle size (mean):	10 µm and 13 µm
Pore size (mean):	400 nm
Functional group:	polyamine
Counter ion:	Cl^-
pH stability:	2.0 - 12.0
Capacity (mg BSA/mL):	70
Small ion capacity:	0.1 eq/L
pKa:	9.4

Analysis of Mouse Ascites Fluid

Columns:	A: TSKgel BioAssist Q, 10 µm, 4.6 mm ID × 5 cm B: Commercial Q-type product A, 5.0 mm ID × 5 cm
Mobile phase:	15 min linear gradient of NaCl from 0 to 1.0 mol/L in 20 mmol/L Tris-HCl buffer, pH 8.0
Flow rate:	1.0 mL/min
Detection:	UV @ 280 nm
Temperature:	25 °C
Injection vol.:	5 µL
Sample:	mouse ascites fluid (3-fold dilution with initial eluent)

For additional applications, please see our applications database.

Performance Enhancement on FPLC System

Columns:	A: TSKgel BioAssist Q, 10 µm, 4.6 mm ID × 5 cm B: Competitor Q, 5.0 mm ID × 5 cm
Mobile phase:	30 min linear gradient from 0 to 1 mol/L NaCl in 20 mmol/L sodium phosphate, pH 8.0
Flow rate:	1.0 mL/min
Detection:	UV @ 280 nm
Samples:	1. conalbumin i. ovalbumin impurity 2. ovalbumin 3. trypsin inhibitor

Dynamic Binding Capacity as function of Protein Molar Mass

Column:	TSKgel BioAssist Q, 10 µm, 4.6 mm ID × 1 cm Conventional Q-type product A, 5.0 mm ID × 1 cm
Mobile phase:	20 mmol/L Tris-HCl buffer, pH 8.0
Flow rate:	0.38 mL/min
Detection:	UV @ 280 nm
Temperature:	25 °C
Samples:	1. trypsin inhibitor, 10 g/L 2. human serum albumin, 10 g/L 3. IgG₁, 2.3 g/L 4. thyroglobulin, 5 g/L