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TSKgel^® Anion Exchange Chromatography Columns

Very efficient chromatography can be achieved with the non-porous TSKgel^® STAT columns due to the novel bonding chemistry and the absence of micro-pores. Applications for the TSKgel^® Q-STAT columns include the separation of: proteins, peptides, low molecular weight nucleic acids, aggregates and charge isomers of monoclonal antibodies, PEGylated proteins, oligo DNA, and siRNA. The TSKgel^® DNA-STAT column is ideal for the analysis of DNA fragments, nucleic acids and nucleotides.

The pore structure and bonding chemistry of TSKgel^® BioAssist Q columns provides high capacity for small to very large molar mass proteins and nucleic acids.

Proteins, peptides, DNA and RNA-derived oligonucleotides, and other nucleic acid fragments are typical samples that are analyzed or isolated on the TSKgel^® DEAE-5PW and TSKgel^® SuperQ-5PW columns.

TSKgel^® DEAE-NPR (non-porous resin) columns are used for the high speed separation and analysis of proteins and poly and oligonucleotides. Applications for the TSKgel^® DNA-NPR (non-porous resin) columns include the high efficiency separation of DNA fragments, PCR products, or plasmids.

TSKgel^® DEAE-2SW and TSKgel^® QAE-2SW (both 5 µm, 12.5 nm) and TSKgel^® DEAE-3SW (10 µm, 25 nm) are available for analyzing smaller molar mass samples such as nucleotides, drug candidates, catecholamines, and small peptides or proteins.

TSKgel^® Sugar AXG columns are packed with 10 µm beads and are used for the gradient separation and analysis of monosaccharides, disaccharides, and sugar alcohols.

For isocratic separation of carbohydrates where lower and constant back pressures may be generated, the 8 µm TSKgel^® Sugar AXI column is optimal.

TSKgel^® SAX columns are used for the separation of isomerized sugars, alcohols, and low molar mass organic acids.

Base Material:	polymethacylate
Particle Size (mean):	10 µm and 13 µm
Pore Size (mean):	400 nm
Functional Group:	polyamine	Visit the TSKgel^® BioAssist Q product page.
Counter Ion:	Cl^-
pH Range:	2.0 - 12.0
Capacity (mg BSA/mL):	70
Small Ion Capacity:	0.1 meq/mL
pKa:	9.4

Base Material:	hydrophilic silica
Particle Size (mean):	5 µm
Pore Size (mean):	12.5 nm
Functional Group:	CH₂CH₂N+(C₂H₅)₃	Visit the TSKgel^® DEAE-2SW product page.
Counter Ion:	H₂PO₄^-
pH Range:	2.0 - 7.5
Capacity (mg BSA/mL):	ND
Small Ion Capacity:	>0.3 meq/mL
pKa:	11.2

Base Material:	hydrophilic silica
Particle Size (mean):	10 µm
Pore Size (mean):	25 nm
Functional Group:	CH₂CH₂N+(C₂H₅)₃	Visit the TSKgel^® DEAE-3SW product page.
Counter Ion:	Cl^-
pH Range:	2.0 - 7.5
Capacity (mg BSA/mL):	ND
Small Ion Capacity:	>0.3 meq/mL
pKa:	11.2

Base Material:	hydroxylated methacrylate polymer
Particle Size (mean):	10, 13 and 20 µm
Pore Size (mean):	100 nm
Functional Group:	CH₂CH₂N+(C₂H₅)₃	Visit the TSKgel^® DEAE-5PW product page.
Counter Ion:	Cl^-
pH Range:	2.0 - 12.0
Capacity (mg BSA/mL):	30
Small Ion Capacity:	0.1 meq/mL
pKa:	11.5

Base Material:	hydroxylated methacrylate polymer
Particle Size (mean):	2.5 µm
Pore Size (mean):	non-porous
Functional Group:	CH₂CH₂N+(C₂H₅)₃	Visit the TSKgel^® DEAE-NPR product page.
Counter Ion:	Cl^-
pH Range:	2.0 - 12.0
Capacity (mg BSA/mL):	5
Small Ion Capacity:	>0.1 meq/mL
pKa:	11.2

Base Material:	hydroxylated methacrylate polymer
Particle Size (mean):	2.5 µm
Pore Size (mean):	non-porous
Functional Group:	proprietary	Visit the TSKgel^® DNA-NPR product page.
Counter Ion:	ClO₄^-
pH Range:	2.0 - 12.0
Capacity (mg BSA/mL):	5
Small Ion Capacity:	0.1 meq/mL
pKa:	11.2

Base Material:	hydrophilic polymer
Particle Size (mean):	5 µm
Pore Size (mean):	non-porous
Functional Group:	quarternary ammonium	Visit the TSKgel^® DNA-STAT product page.
Counter Ion:	Cl
pH Range:	3.0 - 10.0
Static Binding Capacity (mg BSA/g dry gel):	ca. 35 (5 µm)
Small Ion Capacity:	270 µeg/g dry gel
pKa:	10.5
Hardware:	Stainless Steel tubing & fittings, PEEK frits

Base Material:	silica
Particle Size (mean):	5 µm
Pore Size (mean):	12.5 nm
Functional Group:	trimethylamino	Visit the TSKgel^® QAE-2SW product page.
Counter Ion:	H₂PO₄^-
pH Range:	2.0 - 7.5
Capacity (mg BSA/mL):	ND
Small Ion Capacity:	>0.3 meq/mL
pKa:	11.2

Base Material:	hydrophilic polymer
Particle Size (mean):	7 µm and 10 µm
Pore Size (mean):	non-porous
Functional Group:	quarternary ammonium	Visit the TSKgel^® Q-STAT product page.
Counter Ion:	Cl
pH Range:	3.0 - 10.0
Static Binding Capacity (mg BSA/g dry gel):	ca. 35 (7 µm) ca. 20 (10 µm)
Small Ion Capacity:	270 µeg/g dry gel
pKa:	10.5
Hardware:	Stainless Steel tubing & fittings, PEEK frits

Base Material:	PS-DVB polymer
Particle Size (mean):	5 µm
Pore Size (mean):	6.0 nm
Functional Group:	trimethylamine	Visit the TSKgel^® SAX product page.
Counter Ion:	Cl^-
pH Range:	1.0 - 14.0
Capacity (mg BSA/mL):	ND
Small Ion Capacity:	>0.1 meq/mL
pKa:	12.5

Base Material:	PS-DVB polymer
Particle Size (mean):	10 µm
Pore Size (mean):	6.0 nm
Functional Group:	trimethylamino	Visit the TSKgel^® Sugar AXG product page.
Counter Ion:	HBO₃^-
pH Range:	1.0 - 14.0
Capacity (mg BSA/mL):	NA
Small Ion Capacity:	>1.2 meq/mL
pKa:	12.5

Base Material:	PS-DVB polymer
Particle Size (mean):	8 µm
Pore Size (mean):	6.0 nm
Functional Group:	trimethylamino	Visit the TSKgel^® Sugar AXI product page.
Counter Ion:	HBO₃^-
pH Range:	1.0 - 14.0
Capacity (mg BSA/mL):	ND
Small Ion Capacity:	>1.2 meq/mL
pKa:	12.5

Base Material:	polymethacrylate
Particle Size (mean):	10 µm and 13 µm
Pore Size (mean):	100 nm
Functional Group:	trimethylamino	Visit the TSKgel^® SuperQ-5PW product page.
Counter Ion:	Cl^-
pH Range:	2.0 - 12.0
Capacity (mg BSA/mL):	100
Small Ion Capacity:	>0.13 meq/mL
pKa:	12.2

Anion Exchange

TSKgel® Anion Exchange Chromatography Columns

TSKgel^® Anion Exchange Chromatography Columns