Separation of Saccharide Mixture
| Columns: |
TSKgel Sugar AXG, 10 µm,
4.6 mm ID × 15 cm
|
| Mobile phase: |
step gradient: 6 min buffer A, 0.6 mol/L boric acid, pH 7.7; then 27 min buffer B, 0.7 mol/L boric acid, pH 7.25; then 30 min buffer C, 0.7 mol/L boric acid, pH 8.7 |
| Flow rate: |
0.4 mL/min (column and post column reagent solution)
|
| Detection: |
fluorescence; Ex: 331 nm, Em: 383 nm |
| Pressure: |
16kg/cm2
|
| Temperature: |
70 °C (column), 100 °C (post column reactor)
|
| PC reagent: |
2.5% 2-cyanoacetamide solution
|
| Samples: |
disaccharides, 25 nm; monosaccharides, 50 nm:
1. cellobiose 2. maltose 3. lactose 4. rhamnose 5. lyxose 6. ribose
7. mannose 8. fructose 9. arabinose
10. galactose 11. xylose
12. glucose |
|
|
Analysis of Palatinit
| Column: |
TSKgel Sugar AXG, 10 µm,
4.6 mm ID × 15 cm
|
| Mobile phase: |
0.7 mol/L borate buffer, pH 8.6 |
| Flow rate: |
0.8 mL/min |
| Detection: |
RI |
| Temperature: |
65 °C
|
| Injection vol.: |
10 µL
|
| Samples: |
1. alpha-D-glucopyranosyl-1,6-soribitol (GPS)
2. alpha-D-glycopyranosyl-1,6-mannitol (GPM) |
|
For additional applications, please see our applications database.