TSKgel® FcR-IIIA Affinity Chromatography Columns
TSKgel® FcR-IIIA-NPR and FcR-IIIA-5PW, affinity chromatography columns, separate antibodies based on the affinity of their Fc region for a particular Fc receptor (FcγIIIa). The affinity has a direct influence on the mode of action of an antibody. Therefore, the columns are a useful tool for the analysis and optimization of antibodies as well as of their production and storage conditions.
The TSKgel® FcR-IIIA-NPR column is useful for analytical applications and is based on a recombinant FcγIIIa receptor ligand immobilized on a non-porous polymer particle. Applications of this column include fast assessment of biologic activity of monoclonal antibodies, cell-line screenings and lot-to-lot comparisons.
The TSKgel® FcR-IIIA-5PW column is a semi-preparative column with the FcγRIIIA receptor ligand bonded to porous 10 µm polymethacrylate particles. The column can be loaded with up to 5 mg of mAb, allowing material collection in sufficient quantity for in-depth analysis of monoclonal antibody (mAb) glycoforms.