HPLC Columns


TSKgel® FcR-IIIA-NPR is based on a recombinant FcγIIIa receptor ligand immobilized on a non-porous polymer particle. It allows fast assessment of biologic activity of monoclonal antibodies.

FcγIIIa receptor plays a key role in antibody-dependent cell mediated cytotoxicity (ADCC), which is a crucial mechanism of action of anti-tumor therapeutic mAbs. The Fc-glycans of antibodies are known to play an important role in Fc-mediated effector functions. Hence, separation patterns of therapeutic antibodies on TSKgel FcR-IIIA-NPR can be correlated to Fc N-glycans and the ADCC activity of immunoglobulins. Early eluting peaks of TSKgel FcR-IIIA-NPR represent glycoforms with low ADCC activity while late eluting peaks represent glycoforms with high ADCC activity.

The rapid thirty minute separation allows the analysis of large numbers of mAb samples to gain valuable initial information on the distribution of glycoforms and expected ADCC activity. This fast and efficient method can be applied to purified samples and supernatant alike and can therefore be used in many phases of development and production such as cell line screening in early R&D, biosimilar/originator comparison, upstream development and optimization, monitoring of glycoengineering, or lot-to-lot comparison in QC.

NPR stands for Non-Porous Resin.


P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0023513TSKgel FcR-IIIA-NPR5PEEK4.67.52,318


Product Attributes

Base material: polymethacrylate
Particle size: 5 µm
Pore size (mean): non-porous
pH stability: 4.0 - 8.0
Ligand: modified recombinant  FcγRIIIA  


Separation of mAb Glycoforms According to their ADCC Activity

Separation of mAb Glycoforms according to their ADCC Activity


Upstream Monitoring of CHO Cell Culture

Upstream Monitoring of CHO Cell Culture


For additional applications, please see our applications database.



Column Durability - Analysis of CHO Supernatant

    Column Durability - Analysis of CHO Supernatant


Code #DescriptionLiterature Type
AN111 Analyze mAb Glycans Using TSKgel FcR-IIIA-NPR HPLC Column by Top Down Mass SpectrometryApplication Note
AN112 Correlation of FcR Affinity Chromatography with Glycan Pattern and ADCC Activity of a Therapeutic AntibodyApplication Note
AN120 FcR Affinity Chromatography - A New Approach to Assess Glycan Profiles During Cell Line Screening and Upstream DevelopmentApplication Note
AN123 Characterization of mAb Glycoforms on FcγRIIIA-ligand by Analytical and Semi-Preparative ChromatographyApplication Note
DS1243 TSKgel FcR ProductsOperating Conditions and Specifications
LCAT07 TSKgel U/HPLC Columns 2019 Product Guide -- FcR Affinity ColumnsProduct Catalog
PD20 Robustness and Dynamic Range of TSKgel FcR-IIIA-NPR Affinity ColumnPerformance Data
PO47 TSKgel FcR-IIIA-NPR Affinity ColumnProduct Overview
PO49 FcR-IIIA Affinity ChromatographyProduct Overview
TP223 LC-MS Analysis of Monoclonal Antibody Glycoforms using a Novel FcR Receptor Affinity Stationary Phase Paired with High Resolution Mass SpectrometryTechnical Presentation
TP230 An Overview of a Novel Analytical Affinity Chromatography ColumnTechnical Presentation