Applications
The main applications of size exclusion chromatography are desalting of the feedstock, removal of aggregates in a final polishing step and virus purification. In general it can be used to fractionate and purify synthetic and biological polymers, such as proteins, polysaccharides and nucleic acids based on size. The technique is generally combined with others that further separate molecules by other characteristics, such as pI, charge, and affinity for certain compounds and is typically used as a polishing step.
TOYOPEARL® SEC Resins
TOYOPEARL® and TSKgel® chromatography media are polymer based macroporous resins for biopurification. Their rigid polymer backbone has better pressure-flow properties the most other commercially available materials. This simplifies column packing and allows for higher operating velocities thereby increasing throughput and decreasing recycling and sanitization times.
TOYOPEARL® HW and TSKgel® PW size exclusion resins are the foundation for all of Tosoh's ion exchange, hydrophobic interaction, and affinity process media. After an initial polymerization reaction to create the designated particle size and pore size, the resin surface is chemically modified to create the desired final product. Each product is designed for a different molecular weight range and customer feedstock profile.
The table below shows the molar mass separation ranges of each pore size TOYOPEARL® HW size exclusion resin:
