TOYOPEARL HW-65S

TOYOPEARL HW-65S resin is a hydroxylated methacrylic polymer for size exclusion chromatography. TOYOPEARL HW-65S resin has a 100 nm pore size and a 30 µm bead size. Surface hydroxyl groups render this resin very hydrophilic, therefore minimal non-specific adsorption occurs, making the TOYOPEARL HW-65S resin useful for protein separations of biomolecules between 40,000 and 5,000,000 Da in size.

The good mechanical stability of the TOYOPEARL HW-65S resin produces excellent flow characteristics in large industrial size columns (up to 0.3 MPa). TOYOPEARL HW-65S resin is chemically stable from pH 2-14, and it is this stability that allows a constant packing volume over a wide range of salt concentrations and cleaning in place (CIP) with acid or base. Also, this resin can be run at elevated temperatures (4 - 60 °C) and is autoclavable at 121 °C.

If lower resolution is needed, the larger particle TOYOPEARL HW-65F resin can also be used for process SEC.

P/N	Description	Resin Volume	Particle Size (µm)	Price ($)
0019815	TOYOPEARL HW-65S	150 mL	30	641	Inquire
0007467	TOYOPEARL HW-65S	250 mL	30	991	Inquire
0007464	TOYOPEARL HW-65S	500 mL	30	1,474	Inquire
0014688	TOYOPEARL HW-65S	1 L	30	-	Inquire
0008068	TOYOPEARL HW-65S	5 L	30	-	Inquire
0018377	TOYOPEARL HW-65S	50 L	30	-	Inquire

Product Attributes

Pore size (mean):	100 nm
Exclusion limit:	5,000,000 Da
Particle size (mean):	30 µm
Shipping buffer:	20% ethanol
pH stability:	2-13
Stability data:	10 years

Molar Mass Separation Ranges

Polyethylene glycols and oxides	500 - 1 × 10⁶Da
Dextrans	1 × 10⁴ - 1 × 10⁶Da
Globular proteins	4 × 10⁴ - 5 × 10⁶Da

Structure:

Calibration Curves for Globular Proteins

Resins:	A. TOYOPEARL HW-40 B. TOYOPEARL HW-50 C. TOYOPEARL HW-55 D. TOYOPEARL HW-65 E. TOYOPEARL HW-75
Column size:	22 mm ID × 30 cm
Mobile phase:	0.06 mol/L phosphate buffer, pH 7.0, + 0.06 mol/L KCl
Detection:	UV @ 280 nm
Temperature:	ambient
Samples:	protein standards
Legend:	V_e= elution volume V_c= column volume

Polishing Step for Enzyme Purification

Elution profile of protein and activity

Resin:	TOYOPEARL HW-65S
Column size:	16 mm ID × 80 cm
Mobile phase:	20 mol/L acetate buffer with 40% ethylene glycol, pH 4.8
Detection:	UV @ 280 nm
Temperature:	ambient
Sample:	concentrated active fractions from TOYOPEARL Phenyl-650 column

For additional applications, please see our applications database.

Code #	Description	Literature Type
IM02	Instruction Manual for TOYOPEARL and TSKgel PW-type Resins	Instruction Manual
PCAT01	TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Size Exclusion	Product Catalog
RIS0043	TOYOPEARL HW-65	Resin Information Sheet

P/N	Description	Price ($)
0019820	TOYOPEARL SECPAK HP	1,507	Inquire