TOYOPEARL HW-40EC resin is a hydroxylated methacrylic polymer for size exclusion chromatography. TOYOPEARL HW-40EC resin has a 5 nm pore size and a 200 µm bead size. Surface hydroxyl groups render this resin very hydrophilic, therefore minimal non-specific adsorption occurs, making the TOYOPEARL HW-40EC resin useful for protein separations. It is typically used for high throughput desalting and buffer exchange. It also can remove surfactants such as Triton X100 from protein solutions. It is often used for smaller biomolecules with exclusion limits up to 10,000 Da.

The good mechanical stability of the TOYOPEARL HW-40EC resin produces excellent flow characteristics in large industrial size columns (up to 0.3 MPa). TOYOPEARL HW-40EC resin is chemically stable from pH

2-14, and it is this stability that allows a constant packing volume over a wide range of salt concentrations and cleaning in place (CIP) with acid or base. Also, this resin can be run at elevated temperatures (4 - 60 °C) and is autoclavable at 121 °C.

If higher resolution is needed, the smaller particle TOYOPEARL HW-40F and TOYOPEARL HW-40S resins are preferred for process SEC. For desalting, where the resin is used in a filtration mode to remove the target from a buffer, TOYOPEARL HW-40C can also employed in place of TOYOPEARL HW-40EC.