TOYOPEARL HW-75F

TOYOPEARL HW-75F resin is a hydroxylated methacrylic polymer for size exclusion chromatography. TOYOPEARL HW-75F resin has a pore size of > 100 nm and a 45 µm bead size. Surface hydroxyl groups render this resin very hydrophilic, therefore minimal non-specific adsorption occurs, making the TOYOPEARL HW-75F resin useful for protein separations of biomolecules between 500,000 and 50,000,000 Da in size.

The good mechanical stability of the TOYOPEARL HW-75F resin produces excellent flow characteristics in large industrial size columns (up to 0.3 MPa). TOYOPEARL HW-75F resin is chemically stable from pH 2-14, and it is this stability that allows a constant packing volume over a wide range of salt concentrations and cleaning in place (CIP) with acid or base. Also, this resin can be run at elevated temperatures (4 - 60 °C) and is autoclavable at 121 °C.

If higher resolution is needed, the smaller particle TOYOPEARL HW-75S resin can also be used for process SEC.

P/N	Description	Resin Volume	Particle Size (µm)	Price ($)
0019816	TOYOPEARL HW-75F	150 mL	45	322	Inquire
0007469	TOYOPEARL HW-75F	500 mL	45	730	Inquire
0014691	TOYOPEARL HW-75F	1 L	45	-	Inquire
0008072	TOYOPEARL HW-75F	5 L	45	-	Inquire

Product Attributes

Pore size (mean):	>100 nm
Exclusion limit:	50,000,000 Da
Particle size (mean):	45 µm
Shipping buffer:	20% ethanol
pH stability:	2-13
Stability data:	10 years

Molar Mass Separation Ranges

Polyethylene glycols and oxides	4,000 - 5 × 10⁶Da
Dextrans	1 × 10⁵ - 1 × 10⁷Da
Globular proteins	5 × 10⁵ - 5 × 10⁷Da

Structure:

Calibration Curves for Globular Proteins

Resins:	A. TOYOPEARL HW-40 B. TOYOPEARL HW-50 C. TOYOPEARL HW-55 D. TOYOPEARL HW-65 E. TOYOPEARL HW-75
Column size:	22 mm ID × 30 cm
Mobile phase:	0.06 mol/L phosphate buffer, pH 7.0, + 0.06 mol/L KCl
Detection:	UV @ 280 nm
Temperature:	ambient
Samples:	protein standards
Legend:	V_e= elution volume V_c= column volume

Separation of pBR322 DNA

Resin:	TOYOPEARL HW-75F
Column size:	16 mm ID × 130 cm
Mobile phase:	10 mmol/L Tris-HCl, 1 mmol/L EDTA, 0.2 mol/L NaCL, pH 8
Flow rate:	7 cm/hr (0.23 mL/min)
Detection:	UV @ 260 nm
Temperature:	ambient
Sample:	A. crude plasmid DNA extract B. DNA from A C. DNA from hydroxyapatite chromatography

For additional applications, please see our applications database.

Code #	Description	Literature Type
IM02	Instruction Manual for TOYOPEARL and TSKgel PW-type Resins	Instruction Manual
PCAT01	TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Size Exclusion	Product Catalog
RIS0044	TOYOPEARL HW-75	Resin Information Sheet

P/N	Description	Price ($)
0019819	TOYOPEARL SECPAK HMW	522	Inquire