Separation of Nucleotides
| Column: |
TSKgel DEAE-2SW, 5 µm,
4.6 mm ID × 25 cm
|
| Mobile phase: |
A: CH3CN in 0.1 mol/L phosphate, pH 3.0, 20/80
B: CH3CN in 0.5 mol/L phosphate, pH 3.0, 20/80 |
| Gradient: |
30 min linear gradient from buffer A to B |
| Flow rate: |
1.0 mL/min |
| Detection: |
UV @ 260 nm |
| Samples: |
1. AMP 2. IMP 3. GMP 4. ADP 5. ATP |
|
|
Separation of Phosphorothioates
| Column: |
TSKgel DEAE-2SW, 5 µm,
4.6 mm ID × 25 cm
|
| Mobile phase: |
A: 50 mmol/L ammonium acetate
B: 1.5 mol/L ammonium acetate |
| Gradient: |
linear, 0-100% B in 60 minutes |
| Flow rate: |
1 mL/min |
| Detection: |
UV @ 254 nm |
| Temperature: |
25 °C
|
| Samples: |
1. 2 base phosphorothioate oligonucleotide
2. 3 base phosphorothioate oligonucleotide
3. 4 base phosphorothioate oligonucleotide
4. 5 base phosphorothioate oligonucleotide |
|
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