Analysis of High MM RNA
| Columns: |
TSKgel DEAE-5PW, 10 µm,
6 mm ID × 15 cm (custom) |
| Mobile phase: |
300 min linear gradient from 0.3 mol/L to 1.0 mol/L NaCl in 0.1 mol/L Tris-HCl, pH 7.6 |
| Flow rate: |
1.0 mL/min |
| Detection: |
UV @ 260 nm |
| Sample: |
total E. coli RNA |
Detection of HIV-1 PCR-Amplified 130 bp Target
| Column: |
TSKgel DEAE-5PW, 10 µm,
7.5 mm ID × 7.5 cm
|
| Mobile phase: |
A: 25 mmol/L Tris-HCl, 1 mmol/L EDTA, pH 8.0
B: 25 mmol/L Tris-HCl, 1 mmol/L EDTA, pH 8.0, with 1 mol/L NaCl |
| Gradient: |
25-60% B in 30 minutes |
| Flow rate: |
1.0 mL/min |
| Detection: |
UV @ 260 nm |
| Temperature: |
25 °C
|
| Sample: |
pBR322 plasmid |
| Sample load: |
2.5 mg in 1 mL |
|
|
Separation of Monoclonal Antibody
| Column: |
TSKgel DEAE-5PW, 10 µm,
7.5 mm ID x 7.5 cm
|
| Mobile phase: |
A: 20 mmol/L Tris-HCl, pH 8.5
B: A + 0.5 mol/L NaCl
A to B linear gradient (60 min) |
| Flow rate: |
1.0 mL/min |
| Detection: |
UV @ 280 nm |
| Temperature: |
25 °C
|
| Sample: |
anti-human albumin (IgG1), diluted solution of mouse ascites (168 µg in 40 µL) |
|
For additional applications, please see our applications database.