TSKgel SuperQ-5PW

TSKgel SuperQ-5PW columns are packed with porous hydrophilic polymer beads which are modified with a strong anion exchange group. The resulting product exhibits very high protein capacities. TSKgel SuperQ-5PW columns are used for the separation and analysis of proteins, oligonucleotides and other biologically active molecules.

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0018257	TSKgel SuperQ-5PW	10	Stainless Steel	7.5	7.5	1,845	Inquire
0018387	TSKgel SuperQ-5PW	13	Stainless Steel	21.5	15	6,671	Inquire

Product Attributes

Matrix:	polymethacrylate
Particle size (mean):	10 µm and 13 µm
Pore size (mean):	100 nm
Functional group:	trimethylamino
Counter ion:	Cl^-
pH stability:	2.0 - 12.0
Capacity (mg BSA/mL):	100
Small ion capacity:	>0.13 eq/L
pKa:	12.2

Analysis of 16-mer Oligonucleotide

Columns:	TSKgel SuperQ-5PW, 10 µm, 7.5 mm ID × 7.5 cm
Mobile phase:	A: 10 mmol/L NaOH B: 10 mmol/L NaOH with 1 mol/L NaCl
Gradient:	0 min (0% B) 40 min (50% B) 41 min (100% B) 46 min (100% B)
Flow rate:	1 mL/min
Detection:	UV @ 254 nm
Sample:	16-mer morpholine oligonucleotide, AAG AAG AAG AGG GGA G
Sample load:	0.5 O.D. (optical density)

For additional applications, please see our applications database.

Stability of TSKgel SuperQ-5PW columns

Columns:	TSKgel SuperQ-5PW, 10 µm, 7.5 mm ID × 7.5 cm
Mobile phase:	A: 50 mmol/L Tris-HCl, pH 8.6 B: 0.5 mmol/L sodium chloride in 50 mmol/L Tris-HCl, pH 8.6
Gradient:	A-B (60 min)
Flow rate:	1.0 mL/min
Detection:	UV @ 280 nm
Temperature:	25 °C
Injection vol.:	100 µL
Sample load:	each of 1 mg
Samples:	1. ovalbumin 2. trypsin inhibitor
Note:	A. before CIP B. after 15 times (15 days)

Comparison of Various Anion Exchange Columns Under Large Sample Load

Column:	A: TSKgel SuperQ-5PW, 10 µm, 5 mm ID × 5 cm B: Company A, Q type, 5 mm ID × 5 cm C: Company A, perfusion Q type, 6.4 mm ID × 3 cm All column volumes were 1.0 mL
Mobile phase:	A: 50 mmol/L Tris-HCl buffer, pH 8.3 B: A + 0.5 mol/L NaCl A to B linear gradient (60 min)
Flow rate:	0.8 mL/min
Detection:	UV @ 280 nm
Temperature:	25 °C
Injection vol.:	2 mL
Samples:	ovalbumin, 20 mg trypsin inhibitor, 20 mg

Code #	Description	Literature Type
AN040	Selectivity studies in the analytical separation of oligonucleotides using a TSKgel SuperQ-5PW anion exchange column	Application Note
DS1130	TSKgel SuperQ-5PW Products	Operating Conditions and Specifications
IM01	TSKgel Column Instruction Manual	Instruction Manual
LCAT02A	TSKgel U/HPLC Columns 2019 Product Guide -- AIEC Columns	Product Catalog
SR093	Column for High Performance, High-Binding Capacity Ion Exchange Chromatography: TSKgel SuperQ-5PW and Its Applications	Separation Report
TP169	Selectivity studies in the analytical separation of oligonucleotides using anion exchange chromatography	Technical Presentation
TP200	Analytical Separation Of Oligonucleotides Using Strong Anion Exchange and C18 Reversed Phase Chromatography	Technical Presentation

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0018388	TSKgel SuperQ-5PW Guardgel Kit for 7.5 mm ID column	20	Stainless Steel		0	1145