Separation of Theophylline and Metabolites
| Column: |
TSKgel NH2-100, 3 µm,
2.0 mm ID × 15 cm
A: Supernatant of serum deproteinated with 10-fold ACN
B: Supernatant of spiked serum deproteinated with 10-fold ACN |
| Mobile phase: |
A: 0.1 mol/L triethylamine-formic acid, pH 10.0/ACN (5/95)
B: 0.1 mol/L triethylamine-formic acid, pH 10.0/ACN (50/50) |
| Gradient: |
0 min-2 min (0%B) 2-30 min (80%B) 30-32 min(0%B) |
| Flow rate: |
0.25 mL/min |
| Detection: |
UV @ 254 nm |
| Temperature: |
40 °C
|
| Injection vol.: |
10 µL
|
| Samples: |
1. theophylline
2. 3-methylxanthine
3. 1,3-dimethyluric acid
4. 1-methyluric acid
(concentration: 10 mg/L for each) |
|
|
Characterization of Surfactant Profile in Armor All Wash and Wax using 2D-LC with the TSKgel ODS-140HTP and TSKgel NH2-100 Columns
| Column: |
TSKgel ODS-140HTP, 2.3 µm,
2.1 mm ID × 5 cm
TSKgel NH2-100, 3 µm,
2.0 mm ID × 15 cm
|
| Mobile phase: |
A: CH3CN
B: 100 mmol/L ammonium acetate, pH 5.4 |
| Gradient: |
40-60% A, 2.7 minutes, hold at 85% A, 5.4 minutes |
| Flow rate: |
0.5 mL/min |
| Detection: |
UV @ 280 nm, 254 nm, and 210 nm
FLD λex 280 nm, λem 350 nm |
| Temperature: |
30 °C
|
| Injection vol.: |
1 µL
|
| Samples: |
Triton™ X
Triton N
sodium xylenesulfonate
sodium dodecylbenzene sulfonate |
|
For additional applications, please see our applications database.
| TSKgel NH2-100, 3 µm, 2.0 mm ID x 15 cm |
| TSKgel NH2-100, 3 µm, 2.0 mm ID x 15 cm |