Comparison of Glycan Peak Profile of Monoclonal Antibodies
Columns: |
TSKgel Amide-80, 2 µm,
2.0 mm ID × 15 cm
|
Mobile phase: |
A: 200 mmol/L acetic acid + triethylamine, pH 7.3
B: ACN |
Gradient: |
75% B (0-5 min)
75 - 50% (5 - 80 min, linear) |
Flow rate: |
0.5 mL/min |
Detection: |
fluorescence (λex:315 nm,λem: 380 nm |
Injection vol.: |
50 µL |
Samples: |
A: pyridylaminated oligosaccharides released from mAb-1 (mouse)
B: pyridylaminated oligosaccharides released from mAb-2 (human)
C: PA-glucose oligomer (3 - 22 mer) (TaKaRa Bio) |
|
|
Ultra-high Fast Separation with TSKgel
Amide-80, 2 µm Column
Column: |
TSKgel Amide-80, 2 µm,
2.0 mm ID × 5 cm
|
Mobile phase: |
20 mmol/L NH4OAc, pH 4.7 / acetronitrile = 10/90 |
Flow rate: |
1.29 mL/min |
Detection: |
UV @ 254 nm |
Temperature: |
40 °C
|
Injection vol.: |
2 µL
|
Samples: |
1. toluene (1 g/L)
2. theophylline (0.1 g/L)
3. theobromine (0.1 g/L)
4. NPβGlu (0.1 g/L)
5. NPαGlu (0.1 g/L)
6. 2'-deoxyuridine (0.1 g/L)
7. 5-methyluridine (0.1 g/L)
8. uridine (0.1 g/L) |
|
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