Separation of φX174 RF DNA-Hae III Digest (4.5 µg/50 µL)
| Column: |
TSKgel G4000SWXL, 8 µm,
7.8 mm ID × 30 cm |
| Mobile phase: |
0.05 mol/L phosphate buffer, pH 7.0 +
0.3 mol/L NaCl + 1 mmol/L EDTA |
| Flow rate: |
0.15 mL/min
|
| Detection: |
UV @ 260 nm
|
| Temperature: |
25 °C |
|
Separation of Immunoglobulins
| Column: |
TSKgel G4000SWXL, 8 µm,
7.8 mm ID × 30 cm |
| Mobile phase: |
20 mmol/L phosphate buffer + 0.3 mol/L NaCl, ph 7.0
|
| Flow rate: |
0.5 mL/min
|
| Detection: |
UV @ 280 nm
|
| Temperature: |
25 °C
|
| Injection vol.: |
15 µL
|
| Concentration: |
0.17 g/L
|
| Samples: |
1. human IgM (from myeloma,
MW: 970 kDA)
2. human IgA (colostrum, MW: 390 kDa)
3. human IgG (MW: 150 kDa)
4. transferrin (MW: 80 kDA)
5. human serum albumin (MW: 66 kDa)
|
|
For additional applications, please see our applications database.