TSKgel CM-5PW

The TSKgel CM-5PW column is packed with porous hydrophilic polymer beads which are surface modified with carboxymethyl (CM) ligands to provide a weak cation exchange column. It is used for the separation and analysis of proteins, peptides, and other biologically active molecules.

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0013068	TSKgel CM-5PW	10	Stainless Steel	7.5	7.5	1,886	Inquire
0014021	TSKgel CM-5PW	13	Stainless Steel	21.5	15	6,544	Inquire

Product Attributes

Matrix:	polymethacrylate
Particle size (mean):	10 µm
Pore size (mean):	100 nm
Functional group:	-CH₂COO^-
Counter ion:	NA⁺
pH stability:	2.0 - 12.0
Capacity (mg Hb/mL):	45
Small ion capacity:	>0.1 eq/L
pKa:	4.2

Selectivity of Strong and Weak TSKgel Cation Exchange Columns

Columns:	TSKgel SP-5PW and TSKgel CM-5PW, 10 µm, 7.5 mm ID × 7.5 cm
Mobile phase:	60 min linear gradient from 0 mol/L to 0.5 mol/L NaCl in 0.02 mol/L phosphate, pH 7.0
Flow rate:	1.0 mL/min
Detection:	UV @ 280 nm
Samples:	1. trypsinogen 2. ribonuclease A 3. α-chymotrypsinogen 4. cytochrome C 5. lysozyme

For additional applications, please see our applications database.

Code #	Description	Literature Type
DS1033	TSKgel CM-5PW Products	Operating Conditions and Specifications
IM01	TSKgel Column Instruction Manual	Instruction Manual
LCAT02B	TSKgel U/HPLC Columns 2019 Product Guide -- CIEC Columns	Product Catalog
SR074	Separation of Monoclonal Antibodies Using TSKgel HPLC Columns	Separation Report

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0013069	TSKgel CM-5PW Guardgel Kit for 7.5 mm ID column	20	Stainless Steel		0	878

HPLC Columns

TSKgel CM-5PW

Product Attributes

Selectivity of Strong and Weak TSKgel Cation Exchange Columns