TSKgel SP-5PW

TSKgel SP-5PW columns are packed with porous hydrophilic polymer beads which are surface modified with sulfopropyl strong cation exchange groups. They are used for the separation and analysis of proteins, peptides, and other biologically active molecules.

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0018758	TSKgel SP-5PW	10	Stainless Steel	2	7.5	1,419	Inquire
0007161	TSKgel SP-5PW	10	Stainless Steel	7.5	7.5	1,886	Inquire
0007575	TSKgel SP-5PW	13	Stainless Steel	21.5	15	6,544	Inquire

Product Attributes

Matrix:	polymethacrylate
Particle size (mean):	10 µm and 13 µm
Pore size (mean):	100 nm
Functional group:	-(CH₂)₃SO₃^-
Counter ion:	Na⁺
pH stability:	2.0 - 12.0
Capacity (mg Hb/mL):	40
Small ion capacity:	>0.1 eq/L
pKa:	2.3

Semi-preparative Purification of Lipoxidase

Column:	TSKgel SP-5PW, 13 µm, 21.5 mm ID × 15 cm
Mobile phase:	120 min linear gradient from 0 mol/L to 0.5 mol/L Na_sSO₄ in 0.02 mol/L acetate, pH 4.5
Flow rate:	4.0 mL/min
Detection:	UV @ 280 nm
Recovery:	lipoxidase activity collected between the two vertical lines was 84%
Sample:	crude lipoxidase, 200 mg

Separation of Peptide Mixture

Column:	TSKgel SP-5PW, 10 µm, 7.5 mm ID × 7.5 cm
Mobile phase:	30 min linear gradient from 0.02 mol/L to 0.5 mol/L phosphate, pH in 30% acetonitrile
Flow rate:	1.0 mL/min
Detection:	UV @ 220 nm
Injection vol.:	50 µL
Samples:	2 µg each of: 1. oxytocin 2. met-enkephalin 3. TRH 4. α-MSH 5. LH-RH (1 µg) 6. neurotensin 7. α-MSH 8. angiotensin II 9. substance P 10. β-endorphin

For additional applications, please see our applications database.

Selectivity of Strong and Weak TSKgel Cation Exchange Columns

Column:	TSKgel SP-5PW and TSKgel CM-5PW, 10 µm, 7.5 mm ID × 7.5 cm
Mobile phase:	60 min linear gradient from 0 mol/L to 0.5 mol/L NaCl in 0.02 mol/L phosphate, pH 7.0
Flow rate:	1.0 mL/min
Detection:	UV @ 280 nm
Samples:	1. trypsinogen 2. ribonuclease A 3. α-chymotrypsinogen 4. cytochrome C 5. lysozyme

Code #	Description	Literature Type
AN025	Characterization Studies of PEGylated Lysozyme using TSKgel HPLC Columns	Application Note
DS1034	TSKgel SP-5PW Products	Operating Conditions and Specifications
IM01	TSKgel Column Instruction Manual	Instruction Manual
LCAT02B	TSKgel U/HPLC Columns 2019 Product Guide -- CIEC Columns	Product Catalog
SR074	Separation of Monoclonal Antibodies Using TSKgel HPLC Columns	Separation Report
SR097	Application of TSKgel Semi-Micro for High-Sensitivity Analyses	Separation Report
TP135	Characterization and Binding Capacity Studies of PEGylated Lysozyme	Technical Presentation

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0019308	TSKgel Guard Cartridge Holder for 2 mm ID cartridges	0		2	1	506
0042153	TSKgel SP-5PW Guard Cartridge for 2 mm ID column, 3 pk	10	Stainless Steel	2	1	0
0016093	TSKgel SP-5PW Guardgel Kit for 21.5 mm ID column	20	Stainless Steel		0	1350
0007211	TSKgel SP-5PW Guardgel Kit for 7.5 mm ID column	20	Stainless Steel		0	878