Analysis of Purified AAV
| Column: |
TSKgel SP-NPR, 2.5 µm,
4.6 mm ID × 3.5 cm
|
| Mobile phase: |
A. 50 mmol/L HEPES, 1 mmol/L EDTA, 5 mmol/L MgCl, pH 7.5
B: 50 mmol/L HEPES, 1 mmol/L EDTA, 5 mmol/L MgCl, pH 7.5 with 0.5 mol/L NaCl; linear gradient from 20% to 100% B in 10 column volumes |
| Gradient: |
0 min (0% B) 2 min (100% B) |
| Flow rate: |
1 mL/min |
| Detection: |
UV @ 280 nm |
| Sample: |
purified adeno-associated virus |
|
|
pH Gradient Analysis of Hemoglobin A1c
| Column: |
TSKgel SP-NPR, 2.5 µm,
4.6 mm ID × 3.5 cm
|
| Mobile phase: |
A: 0.02 mol/L MES, and 0.02 mol/L HEPES-NaOH, pH 6.0
B: 0.02 mol/L MES, and 0.02 mol/L HEPES-NaOH, pH 8.0 |
| Gradient: |
10 min linear gradient from 32% to 75% buffer B (pH 6.66 to pH 7.43) |
| Flow rate: |
1.5 mL/min |
| Detection: |
VIS @ 415 nm |
| Sample: |
hemoglobin standard |
|
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