Fast Separation of Protein Standards
| Column: |
A: TSKgel SP-STAT, 10 µm,
3.0 mm ID × 3.5 cm
B: ProSwift® SCX-1S Monolith,
4.6 mm ID × 5 cm |
| Mobile phase: |
A. 20 mmol/L sodium acetate, pH 5.0
B: 1.0 mol/L NaCl in mobile phase A, pH 5.0 for column A
1.5 mol/L NaCl in mobile phase A, pH 5.0 for column B |
| Gradient: |
0 min (0% B) 1 min (100% B) |
| Flow rate: |
A: 2.0 mL/min
B: 4.73 mL/min |
| Detection: |
UV @ 280 nm |
| Samples: |
1. α-chymotrypsinogen A
2. cytochrome C
3. lysozyme |
|
|
Monitoring of Reaction Products
| Column: |
TSKgel SP-STAT, 10 µm,
3 mm ID × 3.5 cm
|
| Mobile phase: |
A: 20 mmol/L sodium acetate buffer, pH 5.0
B: 1.0 mol/L NaCl in mobile phase A, pH 5.0 |
| Gradient: |
0 min (0% B) 2 min (100% B) |
| Flow rate: |
2.0 mL/min |
| Detection: |
UV @ 280 nm |
| Sample: |
pegylated β-lactoglobulin |
|
For additional applications, please see our applications database.