TSKgel Amide-80, 5 µm

TSKgel Amide-80, 5 µm columns are packed with spherical silica particles that are covalently bonded with carbamoyl groups. The amide stationary phase provides a unique selectivity under regular normal phase conditions or in the hydrophilic interaction mode of chromatography.

TSKgel Amide-80, 5 µm columns possess superior stability in aqueous/organic solvent systems, an advantage over other hydrophilic stationary phases such as traditional amino-bonded phase columns. In addition, TSKgel Amide-80, 5 µm columns are ideally suited for sensitive LC/MS analysis of water-soluble polar compounds because the high organic content in the mobile phase provides rapid evaporation of solvent during electrospray ionization.

Target applications for the TSKgel Amide-80, 5 µm columns include the analysis of saccharides, glycans, oligosaccharides, peptides, and polar compounds from natural products.

The pore size of the bonded phase is indicated by the number in the product description, in this case TSKgel Amide-80 has 8 nm nominal pore size. The nominal pore size of the starting base silica is 10 nm.

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0020009	TSKgel Amide-80, 5 µm	5	Stainless Steel	1	5	1,015	Inquire
0020010	TSKgel Amide-80, 5 µm	5	Stainless Steel	1	10	1,082	Inquire
0021486	TSKgel Amide-80, 5 µm	5	Stainless Steel	1	15	1,097	Inquire
0021487	TSKgel Amide-80, 5 µm	5	Stainless Steel	1	25	1,146	Inquire
0019694	TSKgel Amide-80, 5 µm	5	Stainless Steel	2	5	1,033	Inquire
0019695	TSKgel Amide-80, 5 µm	5	Stainless Steel	2	10	1,082	Inquire
0019696	TSKgel Amide-80, 5 µm	5	Stainless Steel	2	15	1,097	Inquire
0019697	TSKgel Amide-80, 5 µm	5	Stainless Steel	2	25	1,146	Inquire
0019532	TSKgel Amide-80, 5 µm	5	Stainless Steel	4.6	5	1,186	Inquire
0019533	TSKgel Amide-80, 5 µm	5	Stainless Steel	4.6	10	1,135	Inquire
0013071	TSKgel Amide-80, 5 µm	5	Stainless Steel	4.6	25	1,186	Inquire

Product Attributes

Pore size (silica):	8 nm*
Particle size (mean):	5 µm
pH stability:	2.0-7.5
Functional group:	carbamoyl
Max. temperature (°C):	80
Surface area (m²/g)	450

*The pore size of the bonded phase is indicated by the number in the product description, in this case TSKgel Amide-80 has 8 nm nominal pore size. The nominal pore size of the starting base silica is 10 nm.

Structure:

Separation of β-lactoglobulin Tryptic Digest

Columns:	TSKgel Amide-80, 5 µm, 2.0 mm ID × 15 cm
Mobile phase:	A: 0.1% TFA in H₂O B: 0.1% TFA in ACN
Gradient:	0 min (95%B) 15 min (50%B) 17 min (50%B)
Flow rate:	0.2 mL/min
Injection vol.:	1 µL
Sample:	β-lactoglobulin tryptic digest
Instrument:	Q TRAP (AB Sciex)
Ion Source:	ESI +

Separation of Polyalcohols

Column:	TSKgel Amide-80, 5 µm, 4.6 mm ID × 25 cm
Mobile phase:	ACN/H₂O (75/25)
Flow rate:	1.0 mL/min
Detection:	RI
Temperature:	25 °C
Samples:	1. ethylene glycol, 10 mmol/L, 20 µL 2. glycerin, 10 mmol/L, 20 µL 3. erythritol, 10 mmol/L, 20 µL 4. xylitol, 10 mmol/L 20 µL 5. mannitol, 10 mmol/L µL 6. inositol, 4 mmol/L µL

Separation of Oligosaccharides

Column:	TSKgel Amide-80, 5 µm, 4.6 mm ID × 25 cm
Mobile phase:	ACN/H₂O (55/45)
Flow rate:	1.0 mL/min
Detection:	RI
Temperature:	25 °C
Sample:	2 µL, β-cyclodextrin hydrolysate, 1-7 indicate degree of polymerization (4.6 g/L)

For additional applications, please see our applications database.

Code #	Description	Literature Type
AN001	Separation of Peptides by Hydrophilic Interaction Chromatography Using a TSKgel Amide-80 column	Application Note
AN011	TSKgel Amide-80 Normal Phase Chromatography Columns for the Separation of Saccharides-Now Available in 3µm!	Application Note
DS1052	TSKgel Amide-80 Products	Operating Conditions and Specifications
IM01	TSKgel Column Instruction Manual	Instruction Manual
LCAT04	TSKgel U/HPLC Columns 2019 Product Guide -- HILIC Columns	Product Catalog
PD06	TSKgel Amide-80 Columns for the Analysis of Hydrophilic Compounds	Performance Data
PO09	TSKgel Amide-80 Columns	Product Overview
SR055	Separation of Saccharides Using TSKgel Amide-80, a Packing Material for High-Performance Normal Phase Partition Chromatography (part 1)	Separation Report
SR079	Separation of Saccharides Using TSKgel Amide-80, a Packing Material for High Performance Normal Phase Partition Chromatography (part 2)	Separation Report

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0019308	TSKgel Guard Cartridge Holder for 2 mm ID cartridges	0		2	1	506
0019018	TSKgel Guard Cartridge Holder for 3.2 mm ID cartridges	0		3.2	1.5	507
0021941	TSKgel Amide-80 Guard Cartridge for 2 mm ID, 5 µm columns, 3 pk	5	Stainless Steel	2	1	524
0019010	TSKgel Amide-80 Guard Cartridge for 4.6 mm ID, 5 µm columns, 3 pk	5	Stainless Steel	3.2	1.5	372
0019021	TSKgel Amide-80 Guard Column for 4.6 mm ID, 5 µm columns	5	Stainless Steel	4.6	1	307