Separation of Eight Peptides
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Columns: |
TSKgel Octadecyl-2PW, 5 µm,
4.6 mm ID × 15 cm
|
Mobile phase: |
30 min linear gradient from 0.1% TFA/CH3CN from 90/10 to 30/70 |
Flow rate: |
1.0 mL/min
|
Detection: |
UV @ 215 nm |
Temperature: |
ambient |
Samples: |
1. met-enkephalin
2. bradykinin
3. leu-enkaphalin
4. neurotensin
5. bombesin
6. angiotensin I
7. somatostatin
8. insulin (bovine) |
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