TSKgel Octadecyl-4PW

TSKgel Octadecyl-4PW is based on a large pore, highly cross-linked polymethacrylate base matrix bonded with octadecyl groups. The polymeric matrix provides excellent stability in high pH buffer systems and can withstand rigorous cleaning with either acid or base. The large pore size of TSKgel Octadecyl-4PW columns, 50 nm (500Å), allows unhindered access to proteins and other large molar mass biopolymers. The particle size offerings allow for analytical and semi-preparative scale separations.

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0018755	TSKgel Octadecyl-4PW	7	Stainless Steel	2	15	957	Inquire
0013351	TSKgel Octadecyl-4PW	7	Stainless Steel	4.6	15	910	Inquire
0016257	TSKgel Octadecyl-4PW	13	Stainless Steel	21.5	15	4,802	Inquire

Product Attributes

Pore size (mean):	50 nm
Exclusion limit:	1,000 - 2.0 × 10⁵ Da
Estimated ligand density:	1 eq/L
Particle size (mean):	7.0µm and 13 µm
pH stability:	2.0-12.0
Functional group:	C18 (monomeric bonding chemistry)

Structure:

Comparison of pH Conditions

Column:	TSKgel Octadecyl-4PW, 5 µm, 4.6 mm ID × 15 cm
Mobile phase:	A: 0.2% TFA, pH 1.9 B: 0.05 mol/L phosphate buffer, pH 7.1 C: 0.02 mol/L NH₃, pH 10.8
Gradient:	50 min. linear gradient from 0% to 80% CH₃CN
Flow rate:	1.0 mL/min
Detection:	UV @ 220 nm
Samples:	1. met-enkephalin 2. bradykinin 3. leu-enkephalin 4. neurotensin 5. bombesin 6. angiotensin I 7. somatostatin 8. insulin

For additional applications, please see our applications database.

Code #	Description	Literature Type
DS1039	TSKgel Octadecyl-4PW Products	Operating Conditions and Specifications
IM01	TSKgel Column Instruction Manual	Instruction Manual
LCAT05B	TSKgel U/HPLC Columns 2019 Product Guide -- RPC Columns, Polymer-Based	Product Catalog
SR097	Application of TSKgel Semi-Micro for High-Sensitivity Analyses	Separation Report

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0019308	TSKgel Guard Cartridge Holder for 2 mm ID cartridges	0		2	1	506
0019018	TSKgel Guard Cartridge Holder for 3.2 mm ID cartridges	0		3.2	1.5	507
0042160	TSKgel Octadecyl-4PW Guard Cartridge for 2 mm ID column, 3 pk	7	Stainless Steel	2	1	0
0019008	TSKgel Octadecyl-4PW Guard Cartridge for 4.6 mm ID column, 3 pk	7	Stainless Steel	3.2	1.5	380

HPLC Columns

TSKgel Octadecyl-4PW

Product Attributes

Structure:

Comparison of pH Conditions