Analysis and Recovery of Nanogram Protein Samples
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| Column: |
TSKgel Octadecyl-NPR, 2.5 µm, 4.6 mm ID × 3.5 cm
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| Mobile phase: |
10 min linear gradient from 15% to 80% CH3CN in 5 mmol/L HCIO4 |
| Flow rate: |
1.5 mL/min
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| Detection: |
UV @ 220 nm |
| Samples: |
50 ng each of:
1. ribonuclease A
2. insulin
3. cytochrome C
4. lysozyme
5. transferrin
6. myoglobin |
| Note: |
Blank gradient trace also shown
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