TSKgel Phenyl-5PW RP

TSKgel Phenyl-5PW RP is based on a large pore, highly cross-linked polymethacrylate base matrix bonded with phenyl groups through an ether linkage. The polymeric matrix provides excellent stability in high pH buffer systems and can withstand rigorous cleaning with either acid or base. The large pore size of TSKgel Phenyl-5PW columns, 100 nm (1000Å), allows unhindered access to proteins and other large biopolymers. The TSKgel Phenyl-5PW RP column is structurally similar to the TSKgel Phenyl-5PW column used in hydrophobic interaction chromatography (HIC), but the RP column packing is prepared by bonding a higher density of phenyl groups.

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0018756	TSKgel Phenyl-5PW RP	10	Stainless Steel	2	7.5	856	Inquire
0008043	TSKgel Phenyl-5PW RP	10	Stainless Steel	4.6	7.5	910	Inquire
0016260	TSKgel Phenyl-5PW RP	13	Stainless Steel	21.5	15	4,802	Inquire

Product Attributes

Pore size (mean):	100 nm
Exclusion limit:	1.0 × 10⁶Da
Estimated ligand density:	1 eq/L
Particle size (mean):	10 µm and 13 µm
pH stability:	2.0-12.0
Functional group:	phenyl (monomeric bonding chemistry)

Structure:

Protein Analysis

Column:	TSKgel Phenyl-5PW RP, 10 µm, 4.6 mm ID ×7.5 cm
Mobile phase:	2 min linear gradient from 5% to 20% CH₃CN in 0.05% TFA, followed by (A - 48 min/B - 32 min) linear gradient to (80% A/ 60% B) CH₃CN
Flow rate:	1.0 mL/min
Detection:	UV @ 220 nm
Samples:	lactate dehydrogenase A. 40µg in 100µL B. purity check of fraction collected in part A

For additional applications, please see our applications database.

Chemical Stability

Column:	TSKgel Phenyl-5PW RP, 10 µm, 4.6 mm ID × 7.5 cm
Mobile phase:	60 min linear gradient from 5% to 80% CH₃CN in 0.05% TFA
Flow rate:	1.0 mL/min
Detection:	UV @ 220 nm
Samples:	10 µg each of: 1. ribonuclease A 2. cytochrome C 3. lysozyme 4. bovine serum albumin 5. myoglobin

Code #	Description	Literature Type
DS1040	TSKgel Phenyl-5PW RP Products	Operating Conditions and Specifications
IM01	TSKgel Column Instruction Manual	Instruction Manual
LCAT05B	TSKgel U/HPLC Columns 2019 Product Guide -- RPC Columns, Polymer-Based	Product Catalog
SR097	Application of TSKgel Semi-Micro for High-Sensitivity Analyses	Separation Report

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0019308	TSKgel Guard Cartridge Holder for 2 mm ID cartridges	0		2	1	506
0019018	TSKgel Guard Cartridge Holder for 3.2 mm ID cartridges	0		3.2	1.5	507
0042159	TSKgel Phenyl-5PW RP Guard Cartridge for 2 mm ID column, 3 pk	10	Stainless Steel	2	1	0
0019007	TSKgel Phenyl-5PW RP Guard Cartridge for 4.6 mm ID column, 3 pk	10	Stainless Steel	3.2	1.5	372