Comparison of Standard Protein Separation
| Columns: |
A: TSKgel Protein C4-300, 3 µm, 4.6 mm ID × 15 cm
B: Competitor A, 3.5 µm, 4.6 mm ID × 15 cm |
| Mobile phase: |
A: H2O/CH3CN/TFA = 90/10/0.05 (v/v/v)
B: H2O/CH3CN/TFA = 20/80/0.05 (v/v/v)
|
| Gradient: |
0 min (0%B) 45 min (100%B) |
| Flow rate: |
1.0 mL/min |
| Detection: |
UV @ 210 nm |
| Temperature: |
40 °C
|
| Injection vol.: |
10 µL
|
| Samples: |
1. cytochrome C 2. lysozyme
3. BSA 4. α-chymotrypsoinogen A
5. ovalbumin (each 2 µg/10 µL) |
|
|
High Speed Separation of Proteins
| Column: |
TSKgel Protein C4-300, 3 µm, 4.6 mm ID × 5 cm
|
| Mobile phase: |
A: H2O/CH3CN/TFA = 90/10/0.05 (v/v/v)
B: H2O/CH3CN/TFA = 20/80/0.05
|
| Gradient: |
0 min (0%B) 5 min (100%B)
|
| Flow rate: |
3.0 mL/min 2 |
| Detection: |
UV @ 210 nm
|
| Temperature: |
40 °C
|
| Injection vol.: |
10 µL
|
| Samples: |
1. phenylalanine 2. cytochrome C
3. lysozyme 4. BSA
5. α-chymotrypsoinogen A
6. ovalbumin (each 0.2 g/µL) |
|
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