Separation of SRM 870
| Column: |
TSKgel ODS-100V, 3 µm,
4.6 mm ID × 15 cm
|
| Mobile phase: |
20 mmol/L phosphate buffer,
pH 7.0/MeOH (20/80) |
| Flow rate: |
1.0 mL/min |
| Detection: |
UV @ 254 nm |
| Temperature: |
40 °C
|
| Injection vol.: |
10 µL
|
| Samples: |
1. uracil
2. toluene
3. ethyl benzene
4. quinizarin
5. amitriptyline |
|
|
Rapid Identification of 20 Peptide Fragments
| Column: |
TSKgel ODS-100V, 3 µm,
2.0 mm ID × 15 cm
|
| Mobile phase: |
A: 0.1% TFA in H2O
B: 0.1% TFA in ACN |
| Gradient: |
0 min (10% B) 15 min (70% B)
17 min (70% B) |
| Flow rate: |
0.2 mL/min |
| Injection vol.: |
2 µL
|
| Sample: |
β-lactoglobulin tryptic digest |
| Instrument: |
Q TRAP, ESI+
|
|
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