TSKgel ODS-100Z, 3 µm reversed phase columns are a great choice when a change of selectivity from the TSKgel ODS-100V columns is needed to resolve one or more overlapping pairs. The TSKgel ODS-100Z, 3 µm columns contain a high density monomeric C18 bonded phase for maximum retention and selectivity of small molar mass compounds. Exhaustive endcapping prevents secondary interaction with residual silanol groups.

Containing a high carbon content of 20%, TSKgel ODS-100Z, 3 µm columns exhibit a high stability at both low and high pH. This stability at low pH is important when running peptides and proteins. At low pH conditions, silanol groups get removed first by acid hydrolysis before hydrolysis of the alkyl chains takes place. Because of their high bonded phase surface coverage, the TSKgel ODS-100Z, 3 µm columns can be expected to last longer before showing appreciable changes in retention due to increased silanol interaction.

TSKgel ODS-100Z, 3 µm columns provide longer retention for nonpolar compounds and a slightly higher selectivity for nonpolar compounds, for example when you need to separate homologues series, than the TSKgel ODS-100V columns. Steric selectivity is also higher for TSKgel ODS-100Z, 3 µm columns. This plays a role with complex 3-D molecules, such as aromatic hydrocarbons, steroids, etc.

TSKgel ODS-100Z, 3 µm columns are the workhorse for analysis of small molar mass compounds in life science applications.