Analysis of Hydrophilic Peptides
Columns: |
TSKgel Super-ODS, 2.3 µm,
4.6 mm ID × 5 cm
|
Mobile phase: |
13 mmol/L HCIO4/ACN
Linear gradient from 10% to 50% ACN over 10 minutes |
Flow rate: |
2 mL/min |
Detection: |
UV @ 220 nm, micro-flow cell |
Temperature: |
25 °C
|
Samples: |
1. oxytocin
2. a-endorphin
3. bombesin
4. Leu-enkephalin
5. gamma-endorphin
6. somatostatin
|
All peptides were injected at 0.1 to 0.2 µg each.
|
|
PTC Amino Acids
Column: |
TSKgel Super-ODS, 2.3 µm,
4.6 mm ID × 10 cm
|
Mobile phase: |
A: ACN/50 mmol/L acetate buffer, pH 6.0 = 3/97
B: ACN/H2O = 60/40
|
Flow rate: |
1.5 mL/min |
Detection: |
UV @ 254 nm |
Temperature: |
25 °C
|
Injection vol.: |
5 mL (250 pmol)
|
Samples: |
1. Asp 2. Glu 3. Ser 4. Gly
5. His 6. Arg 7. Thr 8. Ala
9. Pro 10. PTC-NH2 11. Try
12. Val 13. Met 14. Cys 15. Ile
16. Leu 17. Phe 18. Lys |
|
For additional applications, please see our applications database.