Products

HPLC Columns

TSKgel Super-ODS

TSKgel Super-ODS columns are packed with monodispersed 2.3 μm spherical silica particles covalently bonded with octadecyl groups. TSKgel Super-ODS is an excellent choice for small peptides, amino acids, tryptic digests, nucleotides, pharmaceutical molecules, and food/beverage samples.


 
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0020015TSKgel Super-ODS2Stainless Steel15779
0019541TSKgel Super-ODS2Stainless Steel25779
0019542TSKgel Super-ODS2Stainless Steel210801
0018154TSKgel Super-ODS2Stainless Steel4.65829
0018197TSKgel Super-ODS2Stainless Steel4.610862
          

Product Attributes

Pore size : 14 nm
Exclusion limit: 2.0 × 10Da
Endcapped: Yes
Particle size: 2.3 µm
pH stability: 2.0-7.5
Functional group: C18 (polymeric bonding chemistry)
% Carbon: 6

Structure:

RPC_Super-ODS_structure.png

                    

Analysis of Hydrophilic Peptides

RPC_Super-ODS-Fig3B_rev.png

 

Columns: TSKgel Super-ODS, 2.3 µm,
4.6 mm ID × 5 cm

Mobile phase: 13 mmol/L HCIO4/ACN
Linear gradient from 10% to 50% ACN over 10 minutes
Flow rate: 2 mL/min
Detection: UV @ 220 nm, micro-flow cell
Temperature: 25 °C
Samples: 1. oxytocin
2. a-endorphin
3. bombesin
4. Leu-enkephalin
5. gamma-endorphin
6. somatostatin

All peptides were injected at 0.1 to 0.2 µg each.

PTC Amino Acids

RPC_Super-ODS-Fig6B.png

 

Column: TSKgel Super-ODS, 2.3 µm,
4.6 mm ID × 10 cm

Mobile phase: A: ACN/50 mmol/L acetate buffer, pH 6.0 = 3/97
B: ACN/H2O = 60/40
Flow rate: 1.5 mL/min
Detection: UV @ 254 nm
Temperature: 25 °C
Injection vol.: 5 mL (250 pmol)
Samples: 1. Asp 2. Glu 3. Ser 4. Gly
5. His 6. Arg 7. Thr 8. Ala
9. Pro 10. PTC-NH2 11. Try
12. Val 13. Met 14. Cys 15. Ile
16. Leu 17. Phe 18. Lys

 

For additional applications, please see our applications database.

 

Code #DescriptionLiterature Type
AO02 Purification of Nucleic Acids Application Overview
DS1119 TSKgel Super-ODS ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT05A TSKgel U/HPLC Columns 2019 Product Guide -- RPC Columns, Silica-BasedProduct Catalog
PO10 TSKgel Super Series Reversed Phase ColumnsProduct Overview
SR089 TSKgel Super-ODS Packed Columns for Ultra-Fast Reversed Phase Liquid ChromatographySeparation Report
TP101 A Highly Consistent and Chemically Stable Silica-based Octadecylsilane Stationary Phase for High Performance Liquid Chromatography: TSKgel Super-ODS Column for LC/MSTechnical Presentation
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0018206TSKgel Super-ODS;-Octyl;-Phenyl Guardfilter Holder for guard filters0  0243
0019308TSKgel Guard Cartridge Holder for 2 mm ID cartridges0 21506
0018207TSKgel Super-ODS;-Octyl;-Phenyl Guardfilter for 4.6 mm ID columns0Stainless Steel40.4367
0019672TSKgel Super-ODS Guard Cartridge for 2 mm ID columns, 3 pk2Stainless Steel21524