Analysis of Mouse Ascites Fluid
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| Columns: |
TSKgel Super-Octyl, 2.3 µm,
4.6 mm ID × 5 cm
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| Mobile phase: |
A: 13 mmol/L HCIO4
B: 13 mmol/L HCIO4/CH3CN = 20/80
40%B to 100%B in a 1.5 min linear gradient |
| Flow rate: |
2.0 mL/min
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| Detection: |
UV @ 220 nm |
| Samples: |
1. ribonuclease A
2. insulin
3. cytochrome C
4. lysozyme
5. α-lactalbumin
6. myoglobin |
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