Analysis of Puranlukast Hydrate Dry Syrup
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| Columns: |
TSKgel Octyl-80Ts, 5 µm,
4.6 mm ID × 15 cm
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| Mobile phase: |
20 mmol/L KH2PO4/ACN/MeOH = 5/5/1 (v/v/v) |
| Flow rate: |
0.6 mL/min
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| Detection: |
UV/VIS @ 260 nm |
| Temperature: |
25 °C |
| Injection vol.: |
4 µL |
| Samples: |
1. pranlukast hydrate, 0.2 mg/L
2. isoamyl p-oxybenzoate
(4-hydroxybenzoic acid isoamyl ester), 0.2 mg/L |
Sample preparation:
Pranlukast solution: To 400 mg of pranlukast hydrate dry syrup, 10 mL of acetonitrile/dimethyl sulfoxide = 3/1(v/v) was added and shaken vigorously. Solution was centrifuged at 3000 rpm for 5 min. To 1 mL of supernatant, 9 mL of acetonitrile/dimethyl sulfoxide = 3/1(v/v) was added.
Isoamyl p-oxybenzoate solution (IS): To 4.03 mg of isoamyl p-oxybenzoate, 10 mL of acetonitrile/dimethyl sulfoxide = 3/1(v/v) was added and dissolved. 5 mL of both solutions were mixed and applied. Sample: pranlukast hydrate dry syrup
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