Separation of Bradykinins
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Columns: |
TSKgel ODS-120T, 5 µm,
4.6 mm ID × 25 cm
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Mobile phase: |
20% CH3CN in 0.05% TFA |
Flow rate: |
1.0 mL/min
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Detection: |
UV @ 220 nm |
Temperature: |
25 °C |
Samples: |
1. Lys-bradykinin
2. Met-Lys-bradykinin
3. bradykinin
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