TSKgel TMS-250

TSKgel TMS-250 columns contain a unique C1 (TMS) bonded phase. The packing is prepared by monomeric binding of trimethyl silyl groups to a 25 nm (250 Å) pore size spherical silica. Due to the low hydrophobicity of the ligand, excellent recoveries are common even when used with large proteins. Proteins such as adolase (158 kDa) exhibit sharp peaks relative to wide pore C18 columns.

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0007190	TSKgel TMS-250	10	Stainless Steel	4.6	7.5	1,107	Inquire

Product Attributes

Pore size (mean):	25 nm
Exclusion limit:	2.0 × 10⁵Da
Endcapped:	Yes
Particle size:	10 µm
pH stability:	2.0-7.5
Functional group:	C1 (monomeric bonding chemistry)
% Carbon:	5

Structure:

High Resolution Protein Separation

Columns:	TSKgel TMS-250, 10 µm, 4.6 mm ID × 7.5 cm
Mobile phase:	60 min linear gradient from 20% to 95% CH₃CN in 0.05% TFA, pH 2.2
Flow rate:	0.61 mL/min
Detection:	UV @ 220 nm
Samples:	5 µg each of: 1. ribonuclease A 2. cytochrome C 3. lysozyme 4. bovine serum albumin 5. aldolase 6. carbonic anhydrase 7. ovalbumin

For additional applications, please see our applications database.

Code #	Description	Literature Type
DS1067	TSKgel TMS-250 Products	Operating Conditions and Specifications
IM01	TSKgel Column Instruction Manual	Instruction Manual
LCAT05A	TSKgel U/HPLC Columns 2019 Product Guide -- RPC Columns, Silica-Based	Product Catalog
TP188	Separation of Proteins with a Wide Variety of Hydrophobicity using a Reversed Phase 30 nm Pore Size Short Alkyl Chain Column	Technical Presentation

HPLC Columns

TSKgel TMS-250

Product Attributes

Structure:

High Resolution Protein Separation