Separation of Octomers
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| Columns: |
TSKgel OligoDNA-RP, 5 µm,
4.6 mm ID × 5 cm
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| Mobile phase: |
120 min linear gradient from 5% to 25% CH3CN in 0.1 mol/L ammonium acetate, pH 7.0 |
| Flow rate: |
1.0 mL/min
|
| Detection: |
UV @ 260 nm |
| Samples: |
1. linker EcoR I, d(CGAATTCG)
2. Hpa I, d(CGTTAACG)
3. linker EcoR V, d(CGATATCG) |
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For additional applications, please see our applications database.