TOYOPEARL AF-Amino-650M is a reactive resin that requires activation for ligand attachment. The resin may be used to couple ligands using their carboxyl groups through peptide bond formation or aldehyde groups by reductive amination. Aldehyde groups may be present in a carbohydrate or glycoprotein ligand or may be introduced into the ligand by mild, periodate oxidation.
The optimized functional group density of TOYOPEARL AF-Amino-650M (100 μmol/mL) is ideal for coupling of either proteins or low molecular weight ligands. For example, lactose was coupled by reductive alkylation to yield a ligand density of ca. 30 μmol/mL resin.