TSKgel SuperQ-5PW (20)

TSKgel SuperQ-5PW (20) resin is a strong anion exchange resin for biomolecule purification. This resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with quaternary amine (Q) strong anion exchange groups. This resin has a 100 nm pore size and a 20 µm bead size.

TSKgel SuperQ-5PW (20) resin is best used for high resolution process polishing steps and the capture of oligonucleotides from crude harvest. If a product with less resolution is needed, the larger particle sized TSKgel SuperQ-5PW (30) or TOYOPEARL SuperQ-650 resins may be used.

TSKgel SuperQ-5PW resin has high chemical and mechanical stability, allowing cleaning-in-place (CIP) and high flow rates. TSKgel PW resins vary from TOYOPEARL resins by having a higher degree of crosslinking which is necessitated by the higher flow pressures needed when using smaller particles for chromatography. The increased level of crosslinking decreases the number of sites available for ligand immobilization when producing TSKgel PW ion exchange chromatography resins.

A TSKgel PW resin will have a lower dynamic binding capacity than the corresponding TOYOPEARL material, however; chromatographic efficiency, peak width, and resolution are improved because of their smaller particle sizes. In this way they are higher performing chromatography resins than their TOYOPEARL counterparts.

Because similarly functionalized TSKgel PW and TOYOPEARL resin types have the same backbone polymer chemistry, the selectivity for proteins and impurities remains the same.

P/N	Description	Resin Volume	Particle Size (µm)	Price ($)
0043383	TSKgel SuperQ-5PW (20)	25 mL	20	346	Inquire
0018535	TSKgel SuperQ-5PW (20)	250 mL	20	2,276	Inquire
0018546	TSKgel SuperQ-5PW (20)	1 L	20	-	Inquire
0018547	TSKgel SuperQ-5PW (20)	5 L	20	-	Inquire
0021920	TSKgel SuperQ-5PW (20)	50 L	20	-	Inquire

Product Attributes

Base bead:	5000PW
Pore size (mean):	100 nm
Particle size (mean):	20 µm
Ligand type:	strong anion
Ligand pKa:	12.2
DBC:	52 - 88 g/L
Shipping buffer:	20% ethanol
pH stability:	2-13
Stability data:	10 years

Structure:

Purification of Oligonucleotides

1.0 mg load

Resin:	TSKgel SuperQ-5PW (20)
Column size:	6.6 mm ID × 18.5 cm (6.3 mL)
Mobile phase:	Buffer A: 20 mmol/L NaOH Buffer B: 20 mmol/L NaOH, 3.0 mol/L NaCl
Gradient:	50% B (2 CV) 50 - 100% B (15 CV) 100% B (2 CV)
Flow rate:	200 cm/hr (1.14 mL/min)
Detection:	UV @ 254 nm
Sample:	crude phosphorothioate deoxyoligonucleotide
Sample load:	1.0 mg

80% DBC elution peak with fraction purity histogram

Purification of oligonucleotide at 80% DBC

Resin:	TSKgel SuperQ-5PW (20)
Column size:	6.6 mm ID × 18.5 cm (6.3 mL)
Mobile phase:	Buffer A: 20 mmol/L NaOH Buffer B: 20 mmol/L NaOH, 3.0 mol/L NaCl
Gradient:	20% B (2 CV) 20 - 100% B (20 CV) 100% B (2 CV)
Flow rate:	200 cm/hr (1.14 mL/min)
Detection:	UV @ 254 nm
Sample:	crude phosphorothioate deoxyoligonucleotide
Sample load:	235 mg

Oligonucleotide purity and yield from 80% DBC purification

Resin:	crude oligo purity	final oligo purity	% yield
TSKgel SuperQ-5PW (20)	66.5%	96.4%	72.5%

For additional applications, please see our applications database.

Maintains Resolution at High Oligonucleotide Load

Resins:	A & C. TSKgel SuperQ-5PW (20) B & D. Source™ 15Q
Column size:	0.66 cm ID × 15 cm (5.1 mL)
Mobile phase:	Buffer A: 20 mmol/L Tris-HCl + 10 mmol/L EDTA, pH 9.0 Buffer B: 20 mmol/L Tris-HCl + 10 mmol/L EDTA + 1.0 mol/L NaCl, pH 9.0
Flow rate:	250 cm/hr (1.43 mL/min)
Detection:	UV @ 254 nm
Sample:	DNA based oligonucleotides
Sample load:	A & B. 1 mg/column C & D. 20 mg/column
Separation conditions:	Column was washed with 5 CV 100% buffer A followed by 11 mL injection. Column was then washed with 3 CV 100% buffer A followed by 6 CV of linear gradient 35-53 buffer B. Finally, column was washed with 5 CV 100% buffer B.
Fractions:	0.5 mL fractions were taken from peaks of interest and analyzed on a TSKgel DNA-NPR column.

Scale Up or Down using the Same Ligand

Resins:	A. TOYOPEARL SuperQ-650C, 100 µm B. TOYOPEARL SuperQ-650M, 65 µm C. TOYOPEARL SuperQ-650S, 35 µm D. TSKgel SuperQ-5PW (30), 30 µm E. TSKgel SuperQ-5PW (20), 20 µm F. TSKgel SuperQ-5PW, 10 µm
Column size:	7.5 mm ID × 7.5 cm
Mobile phase:	Buffer A: 0.02 mol/L Tris-HCl, pH 8.5 Buffer B: 0.5 mol/L NaCl in buffer A
Gradient:	60 min linear gradient from buffer A to buffer B
Flow rate:	136 cm/hr (1.0 mL/min)
Detection:	UV @ 280 nm
Sample:	mAb in mouse ascites (dilution, × 5)
Load volume:	100 µL

Code #	Description	Literature Type
AN024	Preserving Chromatographic Selectivity While Improving Your Process Purification	Application Note
IM02	Instruction Manual for TOYOPEARL and TSKgel PW-type Resins	Instruction Manual
PCAT02	TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Ion Exchange	Product Catalog
PD09	Purification of DNA-Based Oligonucleotide at 60°C on TSKgel SuperQ-5PW (20) Resin	Performance Data
PD10	Increased Load of DNA-Based Oligonucleotide on TSKgel SuperQ-5PW (20) Resin	Performance Data
RIS0032	TSKgel SuperQ-5PW	Resin Information Sheet
TP121	High Resolution Anion Exchange Chromatography Purification of Oligonucleotides	Technical Presentation
TP136	Purification of oligonucleotides using anion-exchange chromatography	Technical Presentation
TP138	One-step oligonucleotide purifications using anion-exchange chromatography resins	Technical Presentation
TP168	Purification of Oligonucleotides Using High Resolution Strong Anion Exchange Chromatography Resins	Technical Presentation
TP169	Selectivity studies in the analytical separation of oligonucleotides using anion exchange chromatography	Technical Presentation
TP174	Chromatographic Purification of Oligonucleotide-Based Biotherapeutic Products	Technical Presentation

P/N	Description	Price ($)
0045208	SkillPak TSKgel SuperQ-5PW (20) 1 mL columns (qty. 5)	315	Inquire
0045244	SkillPak TSKgel SuperQ-5PW (20) 5 mL column	271	Inquire
0043380	TSKgel IEXPAK PW, 20 µm	1,100	Inquire