TOYOPEARL NH2-750F

TOYOPEARL NH2-750F media is a salt tolerant anion exchange resin capable of aggregate removal in both flow-through and binding and elution modes. The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with proprietary primary amine (NH2) strong anion exchange groups.

TOYOPEARL NH2-750F resin is ideal for process scale applications from the capture of proteins from biological feedstock (mammalian cell culture, plasma, bacterial feedstock, etc.) without dilution to the intermediate or final purification of monoclonal antibodies (mAbs) where aggregates and other impurities are removed from the target of interest.

TOYOPEARL NH2-750F resin is available in one particle size, 45 µm, which corresponds to the F-grade designation.

TOYOPEARL NH2-750F exhibits typical binding capacities in the vicinity of 70 g/L for BSA, maintains high capacity at increased linear velocities, has good pressure-flow characteristics, and will withstand back pressures up to 0.3 MPa.

P/N	Description	Resin Volume	Particle Size (µm)	Price ($)
0023438	TOYOPEARL NH2-750F	100 mL	45	559	Inquire
0023439	TOYOPEARL NH2-750F	250 mL	45	1,041	Inquire
0023440	TOYOPEARL NH2-750F	1 L	45	-	Inquire
0023441	TOYOPEARL NH2-750F	5 L	45	-	Inquire
0023442	TOYOPEARL NH2-750F	50 L	45	-	Inquire

Product Attributes

Base bead:	HW-75
Pore size (mean):	> 100 nm
Particle size (mean):	45 µm
Ligand type:	weak anion
Ligand pKa:	8.5
DBC:	> 70 g/L
Shipping buffer:	20% ethanol
pH stability:	2-13
Stability data (estimated):	10 years

Structure:

Aggregate Removal

Separation of aggregates from IgG₁ monomer

Resin:	TOYOPEARL NH2-750F
Column size:	5 cm ID × 5 cm
Mobile phase:	A: 20 mmol/L Tris-HCl, pH 8.0 B: mobile phase A + 1.0 mol/L NaCl
Gradient:	0 - 100% B (60 min)
Flow rate:	300 cm/hr (1.0 mL/min)
Detection:	UV @ 280 nm
Temperature:	ambient
Sample:	mAb (IgG₁), 0.5 g/L

Flow-through removal of aggregates from mAb monomer

Resin:	TOYOPEARL NH2-750F
Column size:	6.6 mm ID × 6 cm
Mobile phase:	A: 10 mmol/L Tris-HCl, 250 mmol/L NaCl, pH 7.0 B: 1.0 mol/L NaOH (CIP)
Gradient:	isocratic
Flow rate:	300 cm/hr (1.71 mL/min)
Detection:	UV @ 280 nm, conductivity
Temperature:	ambient
Sample:	mAb (IgG₁), 1.0 g/L

For additional applications, please see our applications database.

Alkaline Stability

Pressure-Flow Curves

Resin:	TOYOPEARL NH2-750F
Column size:	4.4 cm ID × 29 cm
Mobile phase:	0.1 mol/L NaCl
Flow rate:	multiple

Salt Tolerance Comparison

Resins:	TOYOPEARL NH2-750F TOYOPEARL Q-600C AR TOYOPEARL GigaCap Q-650M TOYOPEARL SuperQ-650M TOYOPEARL DEAE-650M
Column size:	5 cm ID × 5 cm
Mobile phase:	A: 20 mmol/L Tris-HCl, pH 8.0 B: mobile phase A + 2.0 mol/L NaCl
Gradient:	0 - 100% B (120 min)
Flow rate:	300 cm/hr (1.0 mL/min)
Detection:	UV @ 280 nm
Temperature:	ambient
Sample:	BSA (1.0 g/L)

Code #	Description	Literature Type
AN066	TOYOPEARL NH2-750F Aggregate Removal from Monoclonal Antibodies	Application Note
AN091	TOYOPEARL NH2-750F Flow-through Aggregate Removal from Monoclonal Antibodies	Application Note
AN094	Effective Removal of Endotoxins with AEX Resin TOYOPEARL NH2-750F	Application Note
AN116	Reduce Downstream Processing Costs for mAbs by Switching to a Tosoh 2-Step Platform	Application Note
BR10	Best-In-Class Bioprocessing Resins	Brochure
IM02	Instruction Manual for TOYOPEARL and TSKgel PW-type Resins	Instruction Manual
PCAT02	TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Ion Exchange	Product Catalog
PO39	TOYOPEARL NH2-750F, a Salt Tolerant Anion Exchange Resin	Product Overview
RIS0036	TOYOPEARL NH2-750F	Resin Information Sheet
TP217	Evaluation of Viral Clearance for Monoclonal Antibody Chromatographic Process Steps	Technical Presentation

P/N	Description	Price ($)
OC41MDNH2-750F	Resin Seeker TOYOPEARL NH2-750F 96-well plate kit	-	Inquire
0045226	SkillPak AEX 1 mL column library	341	Inquire
0045228	SkillPak Antibody 1 mL column library	434	Inquire
0045232	SkillPak Best in Class 1 mL column library	430	Inquire
0045266	SkillPak Best in Class 5 mL column library	1,406	Inquire
0045229	SkillPak mAb Platform 1 mL column library	430	Inquire
0045263	SkillPak mAb Platform 5 mL column library	844	Inquire
0045230	SkillPak Salt Tolerant 1 mL column library	341	Inquire
0045264	SkillPak Salt Tolerant 5 mL column library	469	Inquire
0045209	SkillPak TOYOPEARL NH2-750F 1 mL columns (qty. 5)	284	Inquire
0045245	SkillPak TOYOPEARL NH2-750F 5 mL column	235	Inquire
0023443	ToyoScreen NH2-750F, 1 mL (qty. 6)	-	Inquire
0023444	ToyoScreen NH2-750F, 5 mL (qty. 6)	-	Inquire
0045021	ToyoScreen RoboColumns NH2-750F, 200 µL	-	Inquire
0045022	ToyoScreen RoboColumns NH2-750F, 600 µL	-	Inquire