TOYOPEARL DEAE-650S

TOYOPEARL DEAE-650S resin is a weak anion exchanger for biomolecule purification. The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with diethylaminoethyl (DEAE) weak anion exchange groups. TOYOPEARL DEAE-650S has a high degree of chemical and mechanical stability, allowing cleaning-in-place (CIP) and high flow rates.

TOYOPEARL DEAE-650S has a 100 nm pore size and a 35 µm bead size and can be used for high resolution intermediate purification and polishing process steps. If greater resolution is needed, the smaller particle TSKgel DEAE-5PW resins may be used. If lower resolution is desired, the larger particle size TOYOPEARL DEAE-650M or TOYOPEARL DEAE-650C resins can be used. As both the TOYOPEARL DEAE-650 and TSKgel DEAE-5PW resins use the same ligand and backbone chemistry, the selectivity between the two resins will be unchanged

P/N	Description	Resin Volume	Particle Size (µm)	Price ($)
0019804	TOYOPEARL DEAE-650S	25 mL	35	217	Inquire
0007472	TOYOPEARL DEAE-650S	250 mL	35	1,562	Inquire
0014692	TOYOPEARL DEAE-650S	1 L	35	-	Inquire
0007973	TOYOPEARL DEAE-650S	5 L	35	-	Inquire
0021483	TOYOPEARL DEAE-650S	50 L	35	-	Inquire

Product Attributes

Base bead:	HW-65
Pore size (mean):	100 nm
Particle size (mean):	35 µm
Ligand type:	weak anion
Ligand pKa:	11.5
DBC:	25 - 35 g/L
Shipping buffer:	20% ethanol
pH stability:	2-13

Structure:

Blood Proteins

Resins:	1. TOYOPEARL DEAE-650S 2. TOYOPEARL DEAE-650M
Column size:	16 mm ID × 15 cm
Mobile phase:	Buffer A: 50 mmol/L Tris-HCl, pH 8.6 Buffer B: 50 mmol/L Tris-HCl, pH 8.6 + 0.5 mol/L NaCl
Gradient:	linear gradient from buffer A to buffer B in 200 mL total volume
Flow rate:	45 cm/hr (1.5 mL/min)
Detection:	UV @ 280 nm
Temperature:	25 °C
Sample:	human serum

Column:	TSKgel G3000SW, 7.5 mm ID × 30 cm × 2 in series
Mobile phase:	0.1 mol/L phosphate, pH 6.8 + 0.1 mol/L sodium sulfate
Detection:	UV @ 280 nm
Temperature:	25 °C
Samples:	1. crude human serum 2. albumin fraction from TOYOPEARL DEAE-650S 3. albumin fraction from TOYOPEARL DEAE-650M

Kato, Y.; Nakamura, K.; Hashimoto, T. Characterization of TSK-GEL DEAE-Toyopearl 650 Ion Exchanger. J. Chromatogr. 1982, 245, 193-211.

Tryptic Digests

Resin:	TOYOPEARL DEAE-650S
Column size:	6 mm ID × 12 cm
Mobile phase:	pyridine/N-ethyl morpholine
Flow rate:	212 cm/hr (1 mL/min)
Detection:	UV @ 280 nm
Temperature:	ambient
Sample:	enzymatic digest of immunoglobulin L chain

For additional applications, please see our applications database.

Scale Up or Down using the Same Ligand

Resins:	TOYOPEARL DEAE-650C, 100µm TOYOPEARL DEAE-650M, 65 µm TOYOPEARL DEAE-650S, 35 µm TSKgel DEAE-5PW (30), 30µm TSKgel DEAE-5PW (20), 20µm TSKgel DEAE-5PW, 10µm
Column size:	7.5 mm ID × 7.5 cm
Mobile phase:	Buffer A: 0.02 mol/L Tris-HCl, pH 8.5 Buffer B: 0.5 mol/L NaCl in buffer A
Gradient:	60 min linear gradient from buffer A to buffer B
Flow rate:	136 cm/hr (1.0 mL/min)
Detection:	UV @ 280 nm
Sample:	mAb in mouse ascites (dilution, ×5)
Load volume:	100 µL

Code #	Description	Literature Type
IM02	Instruction Manual for TOYOPEARL and TSKgel PW-type Resins	Instruction Manual
PCAT02	TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Ion Exchange	Product Catalog
RIS0021	TOYOPEARL DEAE-650	Resin Information Sheet

P/N	Description	Price ($)
0045113	MiniChrom TOYOPEARL DEAE-650S	-	Inquire
0019817	TOYOPEARL IEXPAK HP	486	Inquire