TOYOPEARL DEAE-650C

TOYOPEARL DEAE-650C resin is a weak anion exchanger for biomolecule purification. The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with diethylaminoethyl (DEAE) weak anion exchange groups. TOYOPEARL DEAE-650C has a high degree of chemical and mechanical stability, allowing cleaning-in-place (CIP) and very high flow rates.

TOYOPEARL DEAE-650C has a 100 nm pore size and a 100 µm bead size and can be used for high throughput capture, intermediate purification, and polishing process steps.

If greater resolution is needed, the smaller particle TOYOPEARL DEAE-650M, TOYOPEARL DEAE-650S, or TSKgel DEAE-5PW resins may be used. As both the TOYOPEARL DEAE-650 and TSKgel DEAE-5PW resins use the same ligand and backbone chemistry, the selectivity between the two resin types will be unchanged.

P/N	Description	Resin Volume	Particle Size (µm)	Price ($)
0007988	TOYOPEARL DEAE-650C	250 mL	100	446	Inquire
0014694	TOYOPEARL DEAE-650C	1 L	100	-	Inquire
0007989	TOYOPEARL DEAE-650C	5 L	100	-	Inquire
0022853	TOYOPEARL DEAE-650C	50 L	100	-	Inquire

Product Attributes

Base bead:	HW-65
Pore size (mean):	100 nm
Particle size (mean):	100 µm
Ligand type:	weak anion
Ligand pKa:	11.5
DBC:	25 - 35 g/L
Shipping buffer:	20% ethanol
pH stability:	2-13

Structure:

Scale Up or Down using the Same Ligand

Resins:	TOYOPEARL DEAE-650C, 100µm TOYOPEARL DEAE-650M, 65 µm TOYOPEARL DEAE-650S, 35 µm TSKgel DEAE-5PW (30), 30µm TSKgel DEAE-5PW (20), 20µm TSKgel DEAE-5PW, 10µm
Column size:	7.5 mm ID × 7.5 cm
Mobile phase:	Buffer A: 0.02 mol/L Tris-HCl, pH 8.5 Buffer B: 0.5 mol/L NaCl in buffer A
Gradient:	60 min linear gradient from buffer A to buffer B
Flow rate:	136 cm/hr (1.0 mL/min)
Detection:	UV @ 280 nm
Sample:	mAb in mouse ascites (dilution, ×5)
Load volume:	100 µL

Code #	Description	Literature Type
IM02	Instruction Manual for TOYOPEARL and TSKgel PW-type Resins	Instruction Manual
PCAT02	TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Ion Exchange	Product Catalog
RIS0021	TOYOPEARL DEAE-650	Resin Information Sheet

P/N	Description	Price ($)
OC41MDAEX-96	Resin Seeker TOYOPEARL AIEX 96-well plate kit	426	Inquire

Process Media

TOYOPEARL DEAE-650C

Product Attributes

Scale Up or Down using the Same Ligand