TOYOPEARL GigaCap DEAE-650M

TOYOPEARL GigaCap DEAE-650M media is a high capacity anion exchange resin optimized for the purification of proteins, including monoclonal antibodies. The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been chemically modified to provide a higher number of anionic binding sites and functionalized with diethyl amino ethyl (DEAE) weak anion exchange groups.

TOYOPEARL GigaCap DEAE-650 resin can be used for high throughput capture and intermediate purification of blood products and other proteins. TOYOPEARL GigaCap DEAE-650 resin is available in one particle size, 75 µm, which corresponds to the M-grade designation.

TOYOPEARL GigaCap DEAE-650M exhibits typical dynamic binding capacities > 156 g/L for BSA, maintains high capacity at increased linear velocities, has good pressure-flow characteristics, and will withstand back pressures up to 0.3 MPa.

TOYOPEARL GigaCap DEAE-650M also has excellent binding and elution kinetics, which results in a more concentrated protein fraction collected during the elution step.

P/N	Description	Resin Volume	Particle Size (µm)	Price ($)
0022865	TOYOPEARL GigaCap DEAE-650M	100 mL	75	524	Inquire
0022866	TOYOPEARL GigaCap DEAE-650M	250 mL	75	975	Inquire
0022867	TOYOPEARL GigaCap DEAE-650M	1 L	75	-	Inquire
0022868	TOYOPEARL GigaCap DEAE-650M	5 L	75	-	Inquire
0022869	TOYOPEARL GigaCap DEAE-650M	50 L	75	-	Inquire

Product Attributes

Base bead:	HW-65
Pore size (mean):	100 nm
Particle size (mean):	75 µm
Ligand type:	weak anion
Ligand pKa:	11.5
DBC:	> 156 g/L
Shipping buffer:	20% ethanol
pH stability:	2-13
Stability data (estimated):	10 years

Structure:

Selectivity Comparison

Resins:	TOYOPEARL GigaCap DEAE-650M TOYOPEARL GigaCap Q-650M Capto DEAE
Column size:	7.5 mm ID × 7.5 cm
Mobile phase:	Buffer A: 50 mmol/L Tris-HCl buffer, pH 8.5 Buffer B: buffer A + 1.0 mol/L NaCl, pH 8.5
Gradient:	120 minutes, 0 - 100% B
Flow rate:	136 cm/hr (1.0 mL/min)
Detection:	UV @ 280 nm
Temperature:	ambient
Injection vol.:	100 µL
Samples:	1. transferrin, 2.9 g/L 2. ovalbumin, 6.5 g/L 3. trypsin inhibitor, 10.0 g/L
Sample load:	1.94 mg total protein

Pressure-Flow Curves

Resin:	TOYOPEARL GigaCap DEAE-650M
Column size:	22 mm ID × 20 cm
Mobile phase:	0.1 mol/L NaCl
Detection:	pressure (MPa)

DBC vs. Flow Rate and Residence Time

Resin:	TOYOPEARL GigaCap DEAE-650M
Column size:	6.0 mm ID × 4 cm
Mobile phase:	Buffer A: 0.05 mol/L Tris-HCl buffer, pH 8.5 Buffer B: mobile phase A + 1.0 mol/L NaCl
Flow rates:	212, 407, 623, and 739 cm/hr (1.0, 1.9, 2.9, 3.5 mL/min)
Detection:	UV @ 280 nm
Sample:	BSA (1.0 g/L)

Code #	Description	Literature Type
IM02	Instruction Manual for TOYOPEARL and TSKgel PW-type Resins	Instruction Manual
PCAT02	TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Ion Exchange	Product Catalog
PD14	TOYOPEARL GigaCap DEAE-650M Preserving Resolution at Increased Protein Loads	Performance Data
PD15	TOYOPEARL GigaCap DEAE-650M Performance Comparison to TOYOPEARL DEAE-650M	Performance Data
PO34	TOYOPEARL GigaCap DEAE-650M	Product Overview
RIS0035	TOYOPEARL GigaCap-DEAE-650M	Resin Information Sheet
TP191	Characterization of a Novel High Capacity Weak Anion Exchange Resin	Technical Presentation

P/N	Description	Price ($)
OC41MDGCDE-650M	Resin Seeker TOYOPEARL GigaCap DEAE-650M 96-well plate kit	-	Inquire
0045226	SkillPak AEX 1 mL column library	341	Inquire
0045210	SkillPak TOYOPEARL GigaCap DEAE-650M 1 mL columns (qty. 5)	284	Inquire
0045246	SkillPak TOYOPEARL GigaCap DEAE-650M 5 mL column	235	Inquire
0022872	ToyoScreen GigaCap DEAE-650M, 1 mL (qty. 6)	-	Inquire
0022873	ToyoScreen GigaCap DEAE-650M, 5 mL (qty. 6)	-	Inquire