TOYOPEARL SP-650S media
is a strong cation exchanger for biomolecule purification. The resin is composed of a base material of hydroxylated
methacrylic polymer beads that has been functionalized with sulfopropyl (S) strong
cation exchange groups. TOYOPEARL SP-650S
resin has a high degree of chemical and mechanical stability, allowing
cleaning-in-place (CIP) and high flow rates. TOYOPEARL SP-650S resin has a 100
nm pore size and a 35 µm bead size.
TOYOPEARL SP-650S resin is best used for high resolution
intermediate purification and polishing process steps. If greater resolution is
needed, the smaller particle TSKgel SP-5PW resins may be used. If lower
resolution is desired, the larger particle TOYOPEARL SP-650M or TOYOPEARL
SP-650C are recommended. As both the TOYOPEARL SP-650 and TSKgel SP-5PW resins
use the same ligand and backbone chemistry, the selectivity between the two
resin types will be unchanged.
TOYOPEARL SP-650S
has a dynamic binding capacity for lysozyme of 50 g/L. TOYOPEARL SP-650S resin maintains capacity at
increased linear velocities, has good pressure-flow characteristics, and will
withstand back pressures up to 0.3 MPa.