TOYOPEARL AF-rProtein A-650F resin is an alkaline resistant protein A resin developed for the capture and purification of monoclonal antibodies.  The resins are composed of a base material of hydroxylated methacrylic polymer beads that have been chemically modified to provide multipoint attachment when functionalized with the protein A ligand.

TOYOPEARL AF-rProtein A-650F utilizes an alkaline resistant recombinant Protein A variant. This non-animal derived ligand is bound to the base bead, Toyopearl HW-65F, using multipoint attachment. This contributes to both the resin's alkaline resistance and its low ligand leakages in the range of 5-25ng/mg IgG. Good pressure-flow characteristics and kinetics contribute to maintaining good capacity, particularly at low residence times.

Toyopearl AF-rProtein A-650F maintains its capacity well after repeated CIP exposure to 0.1mol/L NaOH and has reasonable tolerance to CIP with 0.5mol/L NaOH. TOYOPEARL AF-rProtein A-650F also tolerates exposure to above ambient temperatures and if needed can be autoclaved.

TOYOPEARL AF-rProtein A-650F is available in one particle size, 45 µm, which corresponds to the F-grade designation for TOYOPEARL resins.

TOYOPEARL AF-rProtein A-650F exhibits typical dynamic binding capacities of ≥30 g/L for human IgG at 2 minutes residence time, maintains high capacity at increased linear velocities has good pressure-flow characteristics and will withstand back pressures up to 0.3 MPa.

NOW AVAILABLE - NEW PROTEIN A ELISA KIT optimized for TOYOPEARL AF-rProtein A HC-650F and TOYOPEARL AF-rProtein A-650F resins
Tosoh R40 and R28 Protein A, Mix-N-Go ELISA Kit from Cygnus Technologies, catalog # F910. (https://cygnustechnologies.com/product_detail/tosoh-r40-and-r28-protein-a-mix-n-go-elisa.html)