Separation of Two Proteins
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|
| Resin: |
TOYOPEARL AF-Chelate-650M |
| Column size: |
8 mm ID × 7.5 cm |
| Metal ion: |
Zn+2 |
| Mobile phase: |
Buffer A: 20 mmol/L Tris-HCl, 0.5 mol/L NaCl, pH 8.0
Buffer B: buffer A + 0.2 mol/L glycine |
| Gradient: |
0 - 100% B (120 min) |
| Flow rate: |
60 cm/hr (0.5 mL/min) |
| Detection: |
UV @ 280 nm |
| Sample: |
1. ribonuclease A, 250 µg
2. transferrin, 250 µg |
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