Purification of GH Receptor Protein
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| Resin: |
GH-Formyl TOYOPEARL |
| Column size: |
4.6 mm ID × 15 cm in series with TSKgel 3000SW, 7.6 mm ID × 60cm |
| Mobile phase: |
Buffer A: 50 mmol/L Tris-HCl, 20 mmol/L NaCl, 10 mmol/L MgCl2, 0.3 mmol/L phenylmethylsulfonyl fluoride GH receptor protein, 0.1% Triton™ X-100, pH 7.4
Buffer B: buffer A + 6 mol/L urea |
| Gradient: |
Isocratic elution 100% buffer B |
| Flow rate: |
36 cm/hr for 60 min, then 220 cm/hr |
| Detection: |
UV @ 280 nm, specific binding assay |
| Sample: |
16 mg growth hormone (GH) receptor protein in 6 mL Triton X-100 |
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