TSKgel Alpha-M

The TSKgel Alpha-M mixed bed column has an extended linear calibration range and is suitable for samples with a broad molar mass distribution, as well as samples with unknown molar mass.

This column is packed with rigid, porous, polymer beads which show minimal shrinkage or swelling in polar organic solvents (methanol, acetonitrile, DMSO, isopropanol, THF, and HFIP). The Alpha-M column is stable in a wide variety of organic solvents at concentrations up to 100%.

The main application area for the TSKgel Alpha-M column is the analyses of polymers that are soluble in polar organic solvents greater than 10 x 10⁶ Da. Examples include cellulose derivatives, polyimide, and sodium dodecylsulfate (all in 10 mmol/L LiBr in DMF), cleansing gel in methanol, and degree of saponification of polyvinylalcohol in hexafluoroisopropanol (HFIP).

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0018344	TSKgel Alpha-M	13	Stainless Steel	7.8	30	2,137	Inquire

Product Attributes

Matrix:	hydrophilicmethacrylate
Particle size (mean):	13 µm
Pore size (mean):	mixed bed
Exclusion limits
PEO in H₂O:	1 × 10⁷
PS in DMF with 10 mmol/L LiBr:	>1 × 10⁷
PEG in MeOH with 10 mmol/L LiBr:	>3 × 10⁵

Polyethylene Oxide, Polyethylene Glycol, and Polystyrene Calibration Curves

Columns:	TSKgel Alpha columns, 7.8 mm ID × 30 cm
Mobile phase:	A. H₂O B. MeOH with 10 mmol/L LiBr C. DMF with 10 mmol/L LiBr
Flow rate:	1.0 mL/min
Detection:	RI
Temperature:	A. 25°C B. 25°C C. 40°C
Samples:	A, B. polyethylene glycol (PEG) and polyethylene oxide (PEO) standards C. polystyrene (PS) standards

Analysis of Cellulose Derivatives

Column:	TSKgel Alpha-M, 13 µm, 7.8 mm ID × 30 cm
Mobile phase:	A. DMF with 10 mmol/L LiBr B. MeOH with 10 mmol/L LiBr
Flow rate:	0.5 mL/min
Detection:	RI
Temperature:	40 °C
Injection vol.:	50 µL
Samples:	A. ethyl cellulose, 0.1% B. ethyl hydroxyethyl cellulose, 0.1%

For additional applications, please see our applications database.

Code #	Description	Literature Type
AO01	Gel Filtration Chromatography with TSKgel columns	Application Overview
DS1163	TSKgel Alpha-M Products	Operating Conditions and Specifications
IM01	TSKgel Column Instruction Manual	Instruction Manual
LCAT01C	TSKgel U/HPLC Columns 2019 Product Guide -- SEC, Alpha and SuperAW Columns	Product Catalog
PO11	TSKgel Alpha Series Columns for Size Exclusion Chromatography	Product Overview
SR094	Analyzing Polymers that are Soluble in Polar-Organic Solvents with TSKgel Alpha Series Columns	Separation Report

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0018345	TSKgel Alpha-2500-Alpha-M Guard Column for 7.8 mm ID columns	13	Stainless Steel	6	4	813

HPLC Columns

TSKgel Alpha-M

Product Attributes

Polyethylene Oxide, Polyethylene Glycol, and Polystyrene Calibration Curves

Analysis of Cellulose Derivatives