HPLC Columns

TSKgel Alpha-M

The TSKgel Alpha-M mixed bed column has an extended linear calibration range and is suitable for samples with a broad molar mass distribution, as well as samples with unknown molar mass.

This column is packed with rigid, porous, polymer beads which show minimal shrinkage or swelling in polar organic solvents (methanol, acetonitrile, DMSO, isopropanol, THF, and HFIP). The Alpha-M column is stable in a wide variety of organic solvents at concentrations up to 100%.

The main application area for the TSKgel Alpha-M column is the analyses of polymers that are soluble in polar organic solvents greater than 10 x 106 Da. Examples include cellulose derivatives, polyimide, and sodium dodecylsulfate (all in 10 mmol/L LiBr in DMF), cleansing gel in methanol, and degree of saponification of polyvinylalcohol in hexafluoroisopropanol (HFIP).


P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0018344TSKgel Alpha-M13Stainless Steel7.8302,074

Product Attributes

Matrix: hydrophilicmethacrylate
Particle size (mean): 13 µm
Pore size (mean): mixed bed
Exclusion limits
    PEO in H2O: 1 × 107
    PS in DMF with
    10 mmol/L LiBr:
>1 × 107
    PEG in MeOH with
    10 mmol/L LiBr:
>3 × 10​5




Polyethylene Oxide, Polyethylene Glycol, and Polystyrene Calibration Curves


Columns: TSKgel Alpha columns,
7.8 mm ID × 30 cm
Mobile phase: A. H2O
B. MeOH with 10 mmol/L LiBr
C. DMF with 10 mmol/L LiBr
Flow rate: 1.0 mL/min
Detection: RI
Temperature: A. 25°C  B. 25°C  C. 40°C
Samples: A, B. polyethylene glycol (PEG) and
polyethylene oxide (PEO) standards
C. polystyrene (PS) standards


Analysis of Cellulose Derivatives




Column: TSKgel Alpha-M, 13 µm,
7.8 mm ID × 30 cm
Mobile phase: A. DMF with 10 mmol/L LiBr
B. MeOH with 10 mmol/L LiBr
Flow rate: 0.5 mL/min
Detection: RI
Temperature: 40 °C
Injection vol.: 50 µL
Samples: A. ethyl cellulose, 0.1%
B. ethyl hydroxyethyl cellulose, 0.1%


For additional applications, please see our applications database.


Code #DescriptionLiterature Type
AO01 Gel Filtration Chromatography with TSKgel columns Application Overview
DS1163 TSKgel Alpha-M ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT01C TSKgel U/HPLC Columns 2019 Product Guide -- SEC, Alpha and SuperAW ColumnsProduct Catalog
PO11 TSKgel Alpha Series Columns for Size Exclusion ChromatographyProduct Overview
SR094 Analyzing Polymers that are Soluble in Polar-Organic Solvents with TSKgel Alpha Series ColumnsSeparation Report
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0018345TSKgel Alpha-2500-Alpha-M Guard Column for 7.8 mm ID columns13Stainless Steel64789