HPLC Columns

TSKgel BioAssist G4SWxl

TSKgel BioAssist G4SWxl is the PEEK format of the TSKgel G4000SWxl column. With 8 μm particles and 45 nm pores,  these columns provide the largest exclusion limit and the widest fractionation range within the TSKgel SWxl column line. TSKgel BioAssist G4SWxl columns are an excellent choice for pegylated proteins or glycosylated biomolecules.  The fractionation range is applicable for globular protein samples up to 7 million Da.

Widely considered the premium columns for analyzing biopolymers by GFC,  TSKgel BioAssist G4SWxl columns feature high pore volume per unit column volume, low sample adsorption and excellent column efficiency, all contributing to unsurpassed sample resolution.


P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
20025TSKgel BioAssist G4SWxl8PEEK7.8301,829

Product Attributes

Base material: Silica
Particle size (mean): 8 µm
Pore size (mean): 45 nm
Functional group: Diol
pH stability: 2.5-7.5
Calibration range: 2.0 × 10​4​- 7.0 × 10Da (globular proteins)

Calibration Curves


Column: TSKgel SWXL columns,
7.8 mm ID × 30 cm
Mobile phase: 0.3 mol/L NaCl in 0.1 mol/L sodium phosphate buffer, pH 7.0
Detection: UV @ 220 nm
Samples: 1. thyroglobulin (6.6 ×10Da)
2. IgG (1.56 ×10Da)
3. bovine serum albumin
(6.7 ×10Da)
4. ovalbumin (4.3 ×10Da)
5. peroxidase (4.02 ×10Da)
6. β-lactoglobulin (3.5 ×10Da)
7. myoglobin (1.69 ×10Da)
8. ribonuclease A (1.37 ×10Da)
9. cytochrome C (1.24 ×10Da)
10. glycine tetramer (246 Da)

Code #DescriptionLiterature Type
AO01 Gel Filtration Chromatography with TSKgel columns Application Overview
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT01A TSKgel U/HPLC Columns 2019 Product Guide -- SEC, SW Series ColumnsProduct Catalog
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
18008TSKgel BioAssist G2SWxl-G4SWxl Guard Column for 7.8 mm ID columns7PEEK64646