Analysis of Monclonal Antibody and Aggregates Using a TSKgel Butyl-NPR column
| Column: |
TSKgel Butyl-NPR, 2.5 μm,
4.6 mm ID × 3.5 cm |
| Mobile phase: |
A: 3 mol/L NaCl
B: H2O |
| Gradient: |
0-100% B in 10 min |
| Flow rate: |
1.0 mL/min |
| Detection: |
flourescence; Ex: 280 nm, Em: 348 nm |
| Injection vol.: |
5 μg |
| Sample: |
lgG1 |
|
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