HPLC Columns

TSKgel Butyl-NPR

TSKgel Butyl-NPR columns are packed with non-porous 2.5 µm particles grafted to a polymethacrylate resin.

They are the best choice for high-speed separations with excellent recovery even for more hydrophobic samples. TSKgel Butyl-NPR is particularly useful for proteins and other biotherapeutics separations.


P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0014947TSKgel Butyl-NPR2.5Stainless Steel4.63.51,463
0042168TSKgel Butyl-NPR2.5Stainless Steel4.6101,822


Product Attributes

Pore size (mean): non-porous
Particle size (mean): 2.5 µm
pH stability: 2.0-12.0
Functional group: butyl




Analysis of Monclonal Antibody and Aggregates Using a TSKgel Butyl-NPR column



Column: TSKgel Butyl-NPR, 2.5 μm,
4.6 mm ID × 3.5 cm
Mobile phase: A: 3 mol/L NaCl
B: H2O
Gradient: 0-100% B in 10 min
Flow rate: 1.0 mL/min
Detection: flourescence; Ex: 280 nm, Em: 348 nm
Injection vol.: 5 μg
Sample: lgG1

For additional applications, please see our applications database.

Code #DescriptionLiterature Type
AN109 U/HPLC Workflow Solution for Biosimilar CharacterizationApplication Note
BR09 Solutions for Monoclonal Antibody Analysis and CharacterizationBrochure
DS1038 TSKgel Butyl-NPR ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT03 TSKgel U/HPLC Columns 2019 Product Guide -- HIC ColumnsProduct Catalog
SR074 Separation of Monoclonal Antibodies Using TSKgel HPLC ColumnsSeparation Report
TP164 Detection of protein heterogeneity by HPLCTechnical Presentation
TP181 Detection of protein heterogeneity by HPLC (additional data included)Technical Presentation
TP224 Workflow Solution for the Characterization of Biosimilar using Different Modes of Analytical Chromatography TechniquesTechnical Presentation
TP225 Analysis of Monoclonal Antibody Oxidation Using Hydrophobic Interaction ChromatographyTechnical Presentation
TP228 Characterization of Monoclonal Antibody Heterogeneity from Oxidation and Proteolysis by Analytical Hydrophobic Interaction ChromatographyTechnical Presentation