TSKgel Alpha-2500

The TSKgel Alpha-2500 column is packed with rigid, porous, polymer beads which show minimal shrinkage or swelling in polar organic solvents (methanol, acetonitrile, DMSO, isopropanol, THF, and HFIP). This column is stable in a wide variety of organic solvents at concentrations up to 100%.

The main application area for the TSKgel Alpha-2500 column is the analyses of polymers that are soluble in polar organic solvents up to 10,000 Da. Examples include cellulose derivatives, polyimide, and sodium dodecylsulfate (all in 10 mmol/L LiBr in DMF), cleansing gel in methanol, and degree of saponification of polyvinylalcohol in hexafluoroisopropanol (HFIP).

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0018339	TSKgel Alpha-2500	7	Stainless Steel	7.8	30	2,137	Inquire

Product Attributes

Matrix:	hydrophilic methacrylate
Particle size (mean):	7 µm
Pore size (mean):	2.5 nm
Exclusion limits
PEO in H₂O:	5,000
PS in DMF with 10 mmol/L LiBr:	1 × 10⁴
PEG in MeOH with 10 mmol/L LiBr:	1 × 10⁴

Polyethylene Oxide, Polyethylene Glycol, and Polystyrene Calibration Curves

Columns:	TSKgel Alpha columns, 7.8 mm ID × 30 cm
Mobile phase:	A. H₂O B. MeOH with 10 mmol/L LiBr C. DMF with 10 mmol/L LiBr
Flow rate:	1.0 mL/min
Detection:	RI
Temperature:	A. 25 °C B. 25 °C C. 40 °C
Samples:	A, B. polyethylene glycol (PEG) and polyethylene oxide (PEO) standards C. polystyrene (PS) standards

Analysis of Glyceryl tri(2-ethylhexanoate)

Column:	TSKgel Alpha-2500, 7 μm, 7.8 mm ID × 30 cm
Mobile phase:	MeOH

Flow rate:	0.5 mL/min
Detection:	RI
Temperature:	40 °C
Injection vol.:	50 μL
Samples:	glyceryl tri-2-ethylhexanoate
Sample load:	0.10%

Separation of Cleansing Gel

Column:	TSKgel Alpha-2500, 7 μm, 7.8 mm ID × 30 cm
Mobile phase:	methanol
Flow rate:	0.5 mL/min
Detection:	RI
Temperature:	40 °C
Sample:	cleansing gel: 1. glyceryl monstearate POE (20) 2. glyceryl tri-2-ethylhexanoate 3. glycerin 4. sorbitol

For additional applications, please see our applications database.

Code #	Description	Literature Type
AO01	Gel Filtration Chromatography with TSKgel columns	Application Overview
DS1164	TSKgel Alpha-2500 Products	Operating Conditions and Specifications
IM01	TSKgel Column Instruction Manual	Instruction Manual
LCAT01C	TSKgel U/HPLC Columns 2019 Product Guide -- SEC, Alpha and SuperAW Columns	Product Catalog
PO11	TSKgel Alpha Series Columns for Size Exclusion Chromatography	Product Overview
SR094	Analyzing Polymers that are Soluble in Polar-Organic Solvents with TSKgel Alpha Series Columns	Separation Report

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0018345	TSKgel Alpha-2500-Alpha-M Guard Column for 7.8 mm ID columns	13	Stainless Steel	6	4	813