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HPLC Columns

QC-PAK GFC 300GL

The TSKgel QC-PAK 300GL column is 15 cm in length with 5 μm particles.  Made of glass housing material, this column offers the same resolution in half the time as the 30 cm, 5 μm TSKgel G3000SWxl column.

 

The TSKgel QC-PAK GFC 300 column is an excellent choice for protein and large peptide separations.
 
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0016216QC-PAK GFC 300GL5Glass815-
           

Product Attributes

Base material: Silica
Particle size (mean): 5 µm
Pore size (mean): 25 nm
Functional group: Diol
pH stability: 2.5-7.5
Calibration range: 1.0 × 104 - 5.0 × 105 Da (globular proteins)

Calibration Curves

SEC_G2000SWxl-Calibration-rev.png

Column: TSKgel SWXL  columns,
7.8 mm ID × 30 cm
Mobile phase: 0.3 mol/L NaCl in 0.1 mol/L sodium phosphate buffer, pH 7.0
Detection: UV @ 220 nm
Samples: 1. thyroglobulin (6.6 × 105 Da)
2. IgG (1.56 × 105 Da)
3. bovine serum albumin
(6.7 × 104 Da)
4. ovalbumin (4.3 × 104 Da)
5. peroxidase(4.02 × 104 Da)
6. β-lactoglobulin (3.5 × 104 Da)
7. myoglobin (1.69 × 104 Da)
8. ribonuclease A (1.37 × 104 Da)
9. cytochrome C (1.24 × 104 Da)
10. glycine tetramer (246 Da)

                 

Analysis of Various Proteins


SEC_G3000SWxl-Fig14-rev.png

 

Column: TSKgel QC-PAK 300GL, 5 µm,
8 mm ID × 15 cm
Mobile phase: 0.1 mol/LNa2SO4in 0.1 mol/L phosphate buffer, pH 7.0 and 0.05% NaN3
Flow rate: 1.0 mL/min
Detection: UV @ 220 nm
Samples: 1. thyroglobulin
2. IgG
3. ovalbumin
4. ribonuclease
5. p-aminobenzoic acid

For additional applications, please see our applications database.

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Code #DescriptionLiterature Type
DS1012 QC-PAK TSK GFC 300GL AND 300 ProductsOperating Conditions and Specifications
LCAT01A TSKgel U/HPLC Columns 2019 Product Guide -- SEC, SW Series ColumnsProduct Catalog
 

Tosoh Bioscience LLC
 3604 Horizon Drive, Suite 100
King of Prussia, PA 19406

Phone: 800-366-4875
Fax: 484-805-1277

Email: [email protected]